SPELL - Nematode

Citation	van der Linden AM, Beverly M, Kadener S, Rodriguez J, Wasserman S, Rosbash M, Sengupta P. Genome-wide analysis of light- and temperature-entrained circadian transcripts in Caenorhabditis elegans. PLoS Biol, 2010.
PubMed ID	20967231
Short Description	Genome-wide analysis of light- and temperature-entrained circadian transcripts in Caenorhabditis elegans. GEO Record: GSE23528 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00037695.ce.mr.csv
# of Conditions	89
Full Description	Most organisms have an endogenous circadian clock that is synchronized to environmental signals such as light and temperature. Although circadian rhythms have been described in the nematode Caenorhabditis elegans at the behavioral level, these rhythms appear to be relatively non-robust. Moreover, in contrast to other animal models, no circadian transcriptional rhythms have been identified. Thus, whether this organism contains a bona fide circadian clock remains an open question. Here we use genome-wide expression profiling experiments to identify light- and temperature-entrained oscillating transcripts in C. elegans. These transcripts exhibit rhythmic expression with temperature-compensated 24-h periods. In addition, their expression is sustained under constant conditions, suggesting that they are under circadian regulation. Light and temperature cycles strongly drive gene expression and appear to entrain largely nonoverlapping gene sets. We show that mutations in a cyclic nucleotide-gated channel required for sensory transduction abolish both light- and temperature-entrained gene expression, implying that environmental cues act cell nonautonomously to entrain circadian rhythms. Together, these findings demonstrate circadian-regulated transcriptional rhythms in C. elegans and suggest that further analyses in this organism will provide new information about the evolution and function of this biological clock. Experimental Details: WBPaper00037695:T2_LD_3 WBPaper00037695:T6_LD_3 WBPaper00037695:T10_LD_3 WBPaper00037695:T14_LD_3 WBPaper00037695:T18_LD_3 WBPaper00037695:T22_LD_3 WBPaper00037695:T26_DD_2 WBPaper00037695:T30_DD_2 WBPaper00037695:T34_DD_2 WBPaper00037695:T38_DD_2 WBPaper00037695:T42_DD_2 WBPaper00037695:T46_DD_2 WBPaper00037695:T2_LD_4 WBPaper00037695:T6_LD_4 WBPaper00037695:T10_LD_4 WBPaper00037695:T14_LD_4 WBPaper00037695:T18_LD_4 WBPaper00037695:T22_LD_4 WBPaper00037695:T26_DD_4 WBPaper00037695:T30_DD_4 WBPaper00037695:T34_DD_4 WBPaper00037695:T38_DD_4 WBPaper00037695:T42_DD_4 WBPaper00037695:T46_DD_4 WBPaper00037695:T2_LD_6 WBPaper00037695:T6_LD_6 WBPaper00037695:T10_LD_6 WBPaper00037695:T14_LD_6 WBPaper00037695:T18_LD_6 WBPaper00037695:T22_LD_6 WBPaper00037695:T26_DD_6 WBPaper00037695:T30_DD_6 WBPaper00037695:T34_DD_6 WBPaper00037695:T38_DD_6 WBPaper00037695:T42_DD_6 WBPaper00037695:T46_DD_6 WBPaper00037695:T2_control_4 WBPaper00037695:T6_control_4 WBPaper00037695:T1_control_4 WBPaper00037695:T2_control_6 WBPaper00037695:T1_control_6 WBPaper00037695:T2_WC_1 WBPaper00037695:T6_WC_1 WBPaper00037695:T10_WC_1 WBPaper00037695:T14_WC_1 WBPaper00037695:T18_WC_1 WBPaper00037695:T22_WC_1 WBPaper00037695:T2_WC_2 WBPaper00037695:T6_WC_2 WBPaper00037695:T10_WC_2 WBPaper00037695:T14_WC_2 WBPaper00037695:T18_WC_2 WBPaper00037695:T22_WC_2 WBPaper00037695:T2_WC_3 WBPaper00037695:T6_WC_3 WBPaper00037695:T10_WC_3 WBPaper00037695:T14_WC_3 WBPaper00037695:T18_WC_3 WBPaper00037695:T22_WC_3 WBPaper00037695:T26_CC_3 WBPaper00037695:T30_CC_3 WBPaper00037695:T34_CC_3 WBPaper00037695:T38_CC_3 WBPaper00037695:T42_CC_3 WBPaper00037695:T46_CC_3 WBPaper00037695:T2_WC_4 WBPaper00037695:T6_WC_4 WBPaper00037695:T10_WC_4 WBPaper00037695:T14_WC_4 WBPaper00037695:T18_WC_4 WBPaper00037695:T22_WC_4 WBPaper00037695:T26_CC_4 WBPaper00037695:T30_CC_4 WBPaper00037695:T34_CC_4 WBPaper00037695:T38_CC_4 WBPaper00037695:T42_CC_4 WBPaper00037695:T46_CC_4 WBPaper00037695:T2_WC_6 WBPaper00037695:T6_WC_6 WBPaper00037695:T10_WC_6 WBPaper00037695:T14_WC_6 WBPaper00037695:T18_WC_6 WBPaper00037695:T22_WC_6 WBPaper00037695:T26_CC_6 WBPaper00037695:T30_CC_6 WBPaper00037695:T34_CC_6 WBPaper00037695:T38_CC_6 WBPaper00037695:T42_CC_6 WBPaper00037695:T46_CC_6.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: response to temperature stimulus