| Citation | Kim KW, Wilson TL, Kimble J. GLD-2/RNP-8 cytoplasmic poly(A) polymerase is a broad-spectrum regulator of the oogenesis program. Proc Natl Acad Sci U S A, 2010. |
| PubMed ID | 20855596 |
| Short Description | GLD-2/RNP-8 cytoplasmic poly(A) polymerase is a broad-spectrum regulator of the oogenesis program. GEO Record: GSE23843 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00037611.ce.mr.csv |
| # of Conditions | 21 |
Full Description
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Regulated polyadenylation is a broadly conserved mechanism that controls key events during oogenesis. Pivotal to that mechanism is GLD-2, a catalytic subunit of cytoplasmic poly(A) polymerase (PAP). Caenorhabditis elegans GLD-2 forms an active PAP with multiple RNA-binding partners to regulate diverse aspects of germline and early embryonic development. One GLD-2 partner, RNP-8, was previously shown to influence oocyte fate specification. Here we use a genomic approach to identify transcripts selectively associated with both GLD-2 and RNP-8. Among the 335 GLD-2/RNP-8 potential targets, most were annotated as germline mRNAs and many as maternal mRNAs. These targets include gld-2 and rnp-8 themselves, suggesting autoregulation. Removal of either GLD-2 or RNP-8 resulted in shortened poly(A) tails and lowered abundance of four target mRNAs (oma-2, egg-1, pup-2, and tra-2); GLD-2 depletion also lowered the abundance of most GLD-2/RNP-8 putative target mRNAs when assayed on microarrays. Therefore, GLD-2/RNP-8 appears to polyadenylate and stabilize its target mRNAs. We also provide evidence that rnp-8 influences oocyte development; rnp-8 null mutants have more germ cell corpses and fewer oocytes than normal. Furthermore, RNP-8 appears to work synergistically with another GLD-2-binding partner, GLD-3, to ensure normal oogenesis. We propose that the GLD-2/RNP-8 enzyme is a broad-spectrum regulator of the oogenesis program that acts within an RNA regulatory network to specify and produce fully functional oocytes. Experimental Details: WBPaper00037611:GLD-2_wt-IP_bio_rep1 WBPaper00037611:GLD-2_wt-IP_bio_rep2 WBPaper00037611:GLD-2_wt-IP_bio_rep3 WBPaper00037611:GLD-2_wt-IP_bio_rep4 WBPaper00037611:GLD-2_gld-2(RNAi)-IP_bio_rep1 WBPaper00037611:GLD-2_gld-2(RNAi)-IP_bio_rep2 WBPaper00037611:GLD-2_gld-2(RNAi)-IP_bio_rep3 WBPaper00037611:GLD-2_gld-2(RNAi)-IP_bio_rep4 WBPaper00037611:RNP-8_wt-IP_bio_rep1 WBPaper00037611:RNP-8_wt-IP_bio_rep2 WBPaper00037611:RNP-8_wt-IP_bio_rep3 WBPaper00037611:RNP-8_rnp-8(q784)-IP_bio_rep1 WBPaper00037611:RNP-8_rnp-8(q784)-IP_bio_rep2 WBPaper00037611:RNP-8_rnp-8(q784)-IP_bio_rep3 WBPaper00037611:wt_total_RNA_bio_rep1 WBPaper00037611:wt_total_RNA_bio_rep2 WBPaper00037611:wt_total_RNA_bio_rep3 WBPaper00037611:wt_total_RNA_bio_rep4 WBPaper00037611:gld-2(RNAi)_bio_rep1 WBPaper00037611:gld-2(RNAi)_bio_rep2 WBPaper00037611:gld-2(RNAi)_bio_rep3. |
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