| Citation | Honjoh S, Yamamoto T, Uno M, Nishida E. Signalling through RHEB-1 mediates intermittent fasting-induced longevity in C. elegans. Nature, 2009. |
| PubMed ID | 19079239 |
| Short Description | Signalling through RHEB-1 mediates intermittent fasting-induced longevity in C. elegans. GEO Record: GSE9682 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00032430.ce.mr.csv |
| # of Conditions | 24 |
Full Description
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Dietary restriction is the most effective and reproducible intervention to extend lifespan in divergent species. In mammals, two regimens of dietary restriction, intermittent fasting (IF) and chronic caloric restriction, have proven to extend lifespan and reduce the incidence of age-related disorders. An important characteristic of IF is that it can increase lifespan even when there is little or no overall decrease in calorie intake. The molecular mechanisms underlying IF-induced longevity, however, remain largely unknown. Here we establish an IF regimen that effectively extends the lifespan of Caenorhabditis elegans, and show that the low molecular weight GTPase RHEB-1 has a dual role in lifespan regulation; RHEB-1 is required for the IF-induced longevity, whereas inhibition of RHEB-1 mimics the caloric-restriction effects. RHEB-1 exerts its effects in part by the insulin/insulin growth factor (IGF)-like signalling effector DAF-16 in IF. Our analyses demonstrate that most fasting-induced upregulated genes require RHEB-1 function for their induction, and that RHEB-1 and TOR signalling are required for the fasting-induced downregulation of an insulin-like peptide, INS-7. These findings identify the essential role of signalling by RHEB-1 in IF-induced longevity and gene expression changes, and suggest a molecular link between the IF-induced longevity and the insulin/IGF-like signalling pathway. Experimental Details: WBPaper00032430:N2-controlRNAi-fed_1 WBPaper00032430:N2-controlRNAi-fed_2 WBPaper00032430:N2-controlRNAi-fed_3 WBPaper00032430:N2-controlRNAi-fed_4 WBPaper00032430:N2-controlRNAi-fed_5 WBPaper00032430:N2-controlRNAi-fasting_1 WBPaper00032430:N2-controlRNAi-fasting_2 WBPaper00032430:N2-controlRNAi-fasting_3 WBPaper00032430:N2-controlRNAi-fasting_4 WBPaper00032430:N2-controlRNAi-fasting_5 WBPaper00032430:N2-Cel-RhebRNAi-fed_1 WBPaper00032430:N2-Cel-RhebRNAi-fed_2 WBPaper00032430:N2-Cel-RhebRNAi-fed_3 WBPaper00032430:N2-Cel-RhebRNAi-fed_4 WBPaper00032430:N2-Cel-RhebRNAi-fasting_1 WBPaper00032430:N2-Cel-RhebRNAi-fasting_2 WBPaper00032430:N2-Cel-RhebRNAi-fasting_3 WBPaper00032430:N2-Cel-RhebRNAi-fasting_4 WBPaper00032430:N2-Cel-TORRNAi-fed_1 WBPaper00032430:N2-Cel-TORRNAi-fed_2 WBPaper00032430:N2-Cel-TORRNAi-fed_3 WBPaper00032430:N2-Cel-TORRNAi-fasting_1 WBPaper00032430:N2-Cel-TORRNAi-fasting_2 WBPaper00032430:N2-Cel-TORRNAi-fasting_3. |
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