| Citation | Fox RM, Watson JD, Von Stetina SE, McDermott J, Brodigan TM, Fukushige T, Kraus M, Miller DM. The embryonic muscle transcriptome of Caenorhabditis elegans. Genome Biol, 2007. |
| PubMed ID | 17848203 |
| Short Description | The embryonic muscle transcriptome of Caenorhabditis elegans. GEO Record: GSE8462 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00031003.ce.mr.csv |
| # of Conditions | 25 |
Full Description
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ABSTRACT: BACKGROUND: The force generating mechanism of muscle is evolutionarily ancient; the fundamental structural and functional components of the sarcomere are common to motile animals throughout phylogeny. Recent evidence suggests that the transcription factors that regulate muscle development are also conserved. Thus, a comprehensive description of muscle gene expression in a simple model organism should define a basic muscle transcriptome that is also found in animals with more complex body plans. To this end, we have applied Micro-Array Profiling of C. elegans Cells (MAPCeL) to muscle cell populations extracted from developing C. elegans embryos. RESULTS: Fluorescence Activated Cell Sorting (FACS) was used to isolate myo-3::GFP-positive muscle cells, and their cultured derivatives, from dissociated early C. elegans embryos. Microarray analysis identified 7,070 expressed genes, 1,312 of which are enriched in the myo-3::GFP positive cell population relative to the average embryonic cell. The muscle-enriched gene set was validated by comparisons to known muscle markers, independently derived expression data, and GFP reporters in transgenic strains. These results confirm the utility of MAPCeL for cell type-specific expression profiling and reveal that 60% of these transcripts have human homologs. CONCLUSIONS: This study provides a comprehensive description of gene expression in developing C. elegans embryonic muscle cells. The finding that over half of these muscle-enriched transcripts encode proteins with human homologs suggests that mutant analysis of these genes in C. elegans could reveal evolutionarily conserved models of muscle gene function with ready application to human muscle pathologies. Experimental Details: WBPaper00031003:DM100_myo3_24hr_2 WBPaper00031003:DM102_myo3_24hr_3 WBPaper00031003:DM18_N2_0hr_1 WBPaper00031003:DM19_myo3_0hr_1 WBPaper00031003:DM22_N2_0hr_2 WBPaper00031003:DM32_N2_0hr_3 WBPaper00031003:DM33_myo3_0hr_2 WBPaper00031003:DM53_myo3_0hr_3 WBPaper00031003:DM92_myo3_24hr_1 WBPaper00031003:DMR28_N2_24hr_1 WBPaper00031003:DMR30_N2_24hr_2 WBPaper00031003:DMR32_N2_24hr_3 WBPaper00031003:DMR34_N2_24hr_4 WBPaper00031003:HLH-1_induction_0_hour_A WBPaper00031003:HLH-1_induction_0_hour_B WBPaper00031003:HLH-1_induction_0_hour_C WBPaper00031003:HLH-1_induction_2_hour_A WBPaper00031003:HLH-1_induction_2_hour_B WBPaper00031003:HLH-1_induction_2_hour_C WBPaper00031003:HLH-1_induction_4_hour_A WBPaper00031003:HLH-1_induction_4_hour_B WBPaper00031003:HLH-1_induction_4_hour_C WBPaper00031003:HLH-1_induction_6_hour_A WBPaper00031003:HLH-1_induction_6_hour_B WBPaper00031003:HLH-1_induction_6_hour_C. |
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