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Citation Meyer JN, Boyd WA, Azzam GA, Haugen AC, Freedman JH, Van Houten B. Decline of nucleotide excision repair capacity in aging Caenorhabditis elegans. Genome Biol, 2007.
PubMed ID 17472752
Short Description Decline of nucleotide excision repair capacity in aging Caenorhabditis elegans.
GEO Record: GSE4766 Platform: GPL200
Download gene-centric, log2 transformed data: WBPaper00029334.ce.mr.csv
# of Conditions 8
Full Description 1316625150_help ABSTRACT: BACKGROUND: Although Caenorhabditis elegans is an important model for the study of DNA damage- and repair-related processes such as aging, neurodegeneration and carcinogenesis, DNA repair is poorly characterized in this organism. We adapted a quantitative PCR assay to characterize repair of UVC radiation-induced DNA damage in C elegans, and then tested whether DNA repair rates were affected by age in adults. RESULTS: UVC radiation induced lesions in young adult C elegans with a slope of 0.4-0.5 lesions per 10kb DNA per 100 Joules/m2, in both nuclear and mitochondrial targets. L1 and dauer larvae were >5-fold more sensitive to lesion formation than young adults. Nuclear repair kinetics in a well-expressed nuclear gene were biphasic in non-gravid adult nematodes: a faster, first order (t1/2 ~16 h) phase lasting ~24 h and resulting in removal of ~60% of the photoproducts was followed by a much slower phase. Repair in 10 nuclear DNA regions was 15% and 50% higher in more actively transcribed regions in young and aging adults, respectively. Finally, repair was reduced 30-50% in each of the 10 nuclear regions in older adults. However, this decrease in repair could not be explained by a reduction in expression of nucleotide excision repair genes, and we present a plausible mechanism, based on gene expression data, to explain this decrease. CONCLUSIONS: Repair of UVC-induced DNA damage in C elegans is similar kinetically and genetically to repair in humans. Furthermore, this important repair process slows significantly in aging C elegans, the first whole organism in which this question has been addressed.
Experimental Details:
Tags 1316625150_help
Method: microarray, Species: Caenorhabditis elegans, Topic: aging