|Citation||Meyer JN, Boyd WA, Azzam GA, Haugen AC, Freedman JH, Van Houten B. Decline of nucleotide excision repair capacity in aging Caenorhabditis elegans. Genome Biol, 2007.|
|Short Description||Decline of nucleotide excision repair capacity in aging Caenorhabditis elegans. |
GEO Record: GSE4766 Platform: GPL200
Download gene-centric, log2 transformed data: WBPaper00029334.ce.mr.csv
|# of Conditions||8|
|Full Description||ABSTRACT: BACKGROUND: Although Caenorhabditis elegans is an important model for the study of DNA damage- and repair-related processes such as aging, neurodegeneration and carcinogenesis, DNA repair is poorly characterized in this organism. We adapted a quantitative PCR assay to characterize repair of UVC radiation-induced DNA damage in C elegans, and then tested whether DNA repair rates were affected by age in adults. RESULTS: UVC radiation induced lesions in young adult C elegans with a slope of 0.4-0.5 lesions per 10kb DNA per 100 Joules/m2, in both nuclear and mitochondrial targets. L1 and dauer larvae were >5-fold more sensitive to lesion formation than young adults. Nuclear repair kinetics in a well-expressed nuclear gene were biphasic in non-gravid adult nematodes: a faster, first order (t1/2 ~16 h) phase lasting ~24 h and resulting in removal of ~60% of the photoproducts was followed by a much slower phase. Repair in 10 nuclear DNA regions was 15% and 50% higher in more actively transcribed regions in young and aging adults, respectively. Finally, repair was reduced 30-50% in each of the 10 nuclear regions in older adults. However, this decrease in repair could not be explained by a reduction in expression of nucleotide excision repair genes, and we present a plausible mechanism, based on gene expression data, to explain this decrease. CONCLUSIONS: Repair of UVC-induced DNA damage in C elegans is similar kinetically and genetically to repair in humans. Furthermore, this important repair process slows significantly in aging C elegans, the first whole organism in which this question has been addressed.