| Citation | Troemel ER, Chu SW, Reinke V, Lee SS, Ausubel FM, Kim DH. p38 MAPK regulates expression of immune response genes and contributes to longevity in C. elegans. PLoS Genet, 2006. |
| PubMed ID | 17096597 |
| Short Description | p38 MAPK regulates expression of immune response genes and contributes to longevity in C. elegans. GEO Record: GSE5801 GSE5793 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00028789.ce.mr.csv |
| # of Conditions | 27 |
Full Description
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The PMK-1 p38 mitogen-activated protein kinase pathway and the DAF-2-DAF-16 insulin signaling pathway control Caenorhabditis elegans intestinal innate immunity. pmk-1 loss-of-function mutants have enhanced sensitivity to pathogens, while daf-2 loss-of-function mutants have enhanced resistance to pathogens that requires upregulation of the DAF-16 transcription factor. We used genetic analysis to show that the pathogen resistance of daf-2 mutants also requires PMK-1. However, genome-wide microarray analysis indicated that there was essentially no overlap between genes positively regulated by PMK-1 and DAF-16, suggesting that they form parallel pathways to promote immunity. We found that PMK-1 controls expression of candidate secreted antimicrobials, including C-type lectins, ShK toxins, and CUB-like genes. Microarray analysis demonstrated that 25% of PMK-1 positively regulated genes are induced by Pseudomonas aeruginosa infection. Using quantitative PCR, we showed that PMK-1 regulates both basal and infection-induced expression of pathogen response genes, while DAF-16 does not. Finally, we used genetic analysis to show that PMK-1 contributes to the enhanced longevity of daf-2 mutants. We propose that the PMK-1 pathway is a specific, indispensable immunity pathway that mediates expression of secreted immune response genes, while the DAF-2-DAF-16 pathway appears to regulate immunity as part of a more general stress response. The contribution of the PMK-1 pathway to the enhanced lifespan of daf-2 mutants suggests that innate immunity is an important determinant of longevity. Experimental Details: WBPaper00028789:Celegans_gacA_4hours_RepA WBPaper00028789:Celegans_gacA_4hours_RepB WBPaper00028789:Celegans_gacA_4hours_RepC WBPaper00028789:Celegans_gacA_8hours_RepA WBPaper00028789:Celegans_gacA_8hours_RepB WBPaper00028789:Celegans_gacA_8hours_RepC WBPaper00028789:Celegans_OP50_4hours_RepA WBPaper00028789:Celegans_OP50_4hours_RepB WBPaper00028789:Celegans_OP50_4hours_RepC WBPaper00028789:Celegans_OP50_8hours_RepA WBPaper00028789:Celegans_OP50_8hours_RepB WBPaper00028789:Celegans_OP50_8hours_RepC WBPaper00028789:Celegans_PA14_4hours_RepA WBPaper00028789:Celegans_PA14_4hours_RepB WBPaper00028789:Celegans_PA14_4hours_RepC WBPaper00028789:Celegans_PA14_8hours_RepA WBPaper00028789:Celegans_PA14_8hours_RepB WBPaper00028789:Celegans_PA14_8hours_RepC WBPaper00028789:daf2daf16_RepA WBPaper00028789:daf2daf16_RepB WBPaper00028789:daf2daf16_RepC WBPaper00028789:daf2pmk1_RepA WBPaper00028789:daf2pmk1_RepB WBPaper00028789:daf2pmk1_RepC WBPaper00028789:daf2_RepA WBPaper00028789:daf2_RepB WBPaper00028789:daf2_RepC. |
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