SPELL - Nematode

Citation	Liby P, Pohludka M, Vohanka J, Kostrouchova M, Kostrouch D, Rall JE, Kostrouch Z. BIR-1, the homologue of human Survivin, regulates expression of developmentally active collagen genes in C. elegans. Folia Biol (Praha), 2006.
PubMed ID	17116281
Short Description	BIR-1, the homologue of human Survivin, regulates expression of developmentally active collagen genes in C. elegans. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00028809.ce.mr.csv
# of Conditions	6
Full Description	BIR-1 and Survivin are highly conserved members of the inhibitor of apoptosis protein family that regulate cell division in nematodes and mammals and inhibit apoptosis in mammals. In the C. elegans genome, bir-1 is organized in an operon together with transcription and splicing cofactor CeSKIP (skp-1) and is highly expressed during embryogenesis as well as in non-dividing cells during larval development. Previously we have shown that BIR-1 regulates transcription and development and its loss-of-function phenotype overlaps with loss of function of CeSKIP and nuclear hormone receptor CHR3 (NHR-23). Here we searched for genes whose expression is affected by BIR-1 loss of function using whole-genome microarray experiments and identified several collagen genes as candidate targets of bir-1 inhibition in L1 larval stage. The decreased expression of selected collagen genes in bir-l-inhibited larvae was confirmed by quantitative RT-PCR. Next, we generated transgenic lines expressing bir-1 mRNA under a heat shock-regulated promoter and tested whether bir-1 overexpression has the potential to augment the expression of genes that showed decreased expression in worms treated with bir-1 RNAi. Overexpression of bir-1 resulted in a pronounced increase (2 to 5 times) of the expression of these genes. Our findings support the concept that BIR-1, a protein generally regarded as a mitotic factor, is involved in the regulation of transcription during normal development of C. elegans and has a strong ability to affect transcription of developmentally active genes if overexpressed. Experimental Details: WBPaper00028809:control_rep1 WBPaper00028809:control_rep2 WBPaper00028809:control_rep3 WBPaper00028809:bir-1(RNAi)_rep1 WBPaper00028809:bir-1(RNAi)_rep2 WBPaper00028809:bir-1(RNAi)_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: RNAi gene function study