| Citation | Lockhead D, Schwarz EM, O'Hagan R, Bellotti S, Krieg M, Barr MM, Dunn AR, Sternberg PW, Goodman MB. The tubulin repertoire of C. elegans sensory neurons and its context-dependent role in process outgrowth. Mol Biol Cell, 2016. |
| PubMed ID | 27654945 |
| Short Description | The tubulin repertoire of C. elegans sensory neurons and its context-dependent role in process outgrowth. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00050344.ce.rs.csv |
| # of Conditions | 3 |
Full Description
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Microtubules contribute to many cellular processes, including transport, signaling, and chromosome separation during cell division (Kapitein and Hoogenraad, 2015). They are comprised of -tubulin heterodimers arranged into linear protofilaments and assembled into tubes. Eukaryotes express multiple tubulin isoforms (Gogonea et al., 1999), and there has been a longstanding debate as to whether the isoforms are redundant or perform specialized roles as part of a tubulin code (Fulton and Simpson, 1976). Here, we use the well-characterized touch receptor neurons (TRNs) of Caenorhabditis elegans to investigate this question, through genetic dissection of process outgrowth both in vivo and in vitro With single-cell RNA-seq, we compare transcription profiles for TRNs with those of two other sensory neurons, and present evidence that each sensory neuron expresses a distinct palette of tubulin genes. In the TRNs, we analyze process outgrowth and show that four tubulins (tba-1, tba-2, tbb-1, and tbb-2) function partially or fully redundantly, while two others (mec-7 and mec-12) perform specialized, context-dependent roles. Our findings support a model in which sensory neurons express overlapping subsets of tubulin genes whose functional redundancy varies between cell types and in vivo and in vitro contexts. Experimental Details: RNASeq.elegans.WBStrain00005555.WBls:0000002.Hermaphrodite.WBbt:0003903.SRP074511.SRX1746473 RNASeq.elegans.WBStrain00035051.WBls:0000038.Hermaphrodite.WBbt:0005490.SRP074511.SRX1746482 RNASeq.elegans.WBStrain00005557.WBls:0000002.Hermaphrodite.WBbt:0005662.SRP074511.SRX1746483. |
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