| Citation | Cui Y, McBride SJ, Boyd WA, Alper S, Freedman JH. Toxicogenomic analysis of Caenorhabditis elegans reveals novel genes and pathways involved in the resistance to cadmium toxicity. Genome Biol, 2007. |
| PubMed ID | 17592649 |
| Short Description | Toxicogenomic analysis of Caenorhabditis elegans reveals novel genes and pathways involved in the resistance to cadmium toxicity. GEO Record: GSE7535 Platform: GPL2875 Download gene-centric, log2 transformed data: WBPaper00030811.ce.mr.csv |
| # of Conditions | 36 |
Full Description
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ABSTRACT: BACKGROUND: Exposure to cadmium is associated with a variety of human diseases. At low concentrations, cadmium activates the transcription of stress-responsive genes, which can prevent or repair the adverse effects caused by this metal. RESULTS: Using C. elegans, 290 genes were identified that are differentially expressed ([greater than or equal to]1.5-fold) following a 4 or 24 hour exposure to cadmium. Several of these genes are known to be involved in metal detoxification, including mtl-1, mtl-2, cdr-1 and ttm-1, confirming the efficacy of the study. The majority, however, were not previously associated with metal-responsiveness and are novel. Gene Ontology analysis mapped these genes to cellular/ion trafficking, metabolic enzymes and proteolysis categories. RNA interference-mediated inhibition of 50 cadmium-responsive genes resulted in an increased sensitivity to cadmium toxicity, demonstrating that these genes are involved in the resistance to cadmium toxicity. Several functional protein interacting networks were identified by interactome analysis. Within one network, the signaling protein KEL-8 was identified. Kel-8 protects C. elegans from cadmium toxicity in a mek-1 (MAPKK)-dependent manner. CONCLUSIONS: Because many C. elegans genes and signal transduction pathways are evolutionarily conserved, these results may contribute to the understanding of the functional roles of various genes in cadmium toxicity in higher organisms. Experimental Details: WBPaper00030811:C.elegans_Cd_24h_rep1 WBPaper00030811:C.elegans_Cd_24h_rep10 WBPaper00030811:C.elegans_Cd_24h_rep11 WBPaper00030811:C.elegans_Cd_24h_rep12 WBPaper00030811:C.elegans_Cd_24h_rep13 WBPaper00030811:C.elegans_Cd_24h_rep14 WBPaper00030811:C.elegans_Cd_24h_rep15 WBPaper00030811:C.elegans_Cd_24h_rep16 WBPaper00030811:C.elegans_Cd_24h_rep17 WBPaper00030811:C.elegans_Cd_24h_rep18 WBPaper00030811:C.elegans_Cd_24h_rep2 WBPaper00030811:C.elegans_Cd_24h_rep3 WBPaper00030811:C.elegans_Cd_24h_rep4 WBPaper00030811:C.elegans_Cd_24h_rep5 WBPaper00030811:C.elegans_Cd_24h_rep6 WBPaper00030811:C.elegans_Cd_24h_rep7 WBPaper00030811:C.elegans_Cd_24h_rep8 WBPaper00030811:C.elegans_Cd_24h_rep9 WBPaper00030811:C.elegans_Cd_4h_rep1 WBPaper00030811:C.elegans_Cd_4h_rep10 WBPaper00030811:C.elegans_Cd_4h_rep11 WBPaper00030811:C.elegans_Cd_4h_rep12 WBPaper00030811:C.elegans_Cd_4h_rep13 WBPaper00030811:C.elegans_Cd_4h_rep14 WBPaper00030811:C.elegans_Cd_4h_rep15 WBPaper00030811:C.elegans_Cd_4h_rep16 WBPaper00030811:C.elegans_Cd_4h_rep17 WBPaper00030811:C.elegans_Cd_4h_rep18 WBPaper00030811:C.elegans_Cd_4h_rep2 WBPaper00030811:C.elegans_Cd_4h_rep3 WBPaper00030811:C.elegans_Cd_4h_rep4 WBPaper00030811:C.elegans_Cd_4h_rep5 WBPaper00030811:C.elegans_Cd_4h_rep6 WBPaper00030811:C.elegans_Cd_4h_rep7 WBPaper00030811:C.elegans_Cd_4h_rep8 WBPaper00030811:C.elegans_Cd_4h_rep9. |
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