SPELL - Nematode

Citation	Kosmaczewski SG, Edwards TJ, Han SM, Eckwahl MJ, Meyer BI, Peach S, Hesselberth JR, Wolin SL, Hammarlund M. The RtcB RNA ligase is an essential component of the metazoan unfolded protein response. EMBO Rep, 2014.
PubMed ID	25366321
Short Description	The RtcB RNA ligase is an essential component of the metazoan unfolded protein response. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00045971.ce.rs.csv
# of Conditions	4
Full Description	RNA ligation can regulate RNA function by altering RNA sequence, structure and coding potential. For example, the function of XBP1 in mediating the unfolded protein response requires RNA ligation, as does the maturation of some tRNAs. Here, we describe a novel in vivo model in Caenorhabditis elegans for the conserved RNA ligase RtcB and show that RtcB ligates the xbp-1 mRNA during the IRE-1 branch of the unfolded protein response. Without RtcB, protein stress results in the accumulation of unligated xbp-1 mRNA fragments, defects in the unfolded protein response, and decreased lifespan. RtcB also ligates endogenous pre-tRNA halves, and RtcB mutants have defects in growth and lifespan that can be bypassed by expression of pre-spliced tRNAs. In addition, animals that lack RtcB have defects that are independent of tRNA maturation and the unfolded protein response. Thus, RNA ligation by RtcB is required for the function of multiple endogenous target RNAs including both xbp-1 and tRNAs. RtcB is uniquely capable of performing these ligation functions, and RNA ligation by RtcB mediates multiple essential processes in vivo. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP047464.SRX709649 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP047464.SRX709650 RNASeq.elegans.WBStrain00006736.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP047464.SRX709651 RNASeq.elegans.WBStrain00006736.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP047464.SRX709652.
Tags	Method: RNAseq, Species: Caenorhabditis elegans, Topic: response to unfolded protein