| Citation | Washburn MC, Kakaradov B, Sundararaman B, Wheeler E, Hoon S, Yeo GW, Hundley HA. The dsRBP and inactive editor ADR-1 utilizes dsRNA binding to regulate A-to-I RNA editing across the C. elegans transcriptome. Cell Rep, 2014. |
| PubMed ID | 24508457 |
| Short Description | The dsRBP and inactive editor ADR-1 utilizes dsRNA binding to regulate A-to-I RNA editing across the C. elegans transcriptome. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00044827.ce.rs.csv |
| # of Conditions | 8 |
Full Description
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Inadequate adenosine-to-inosine editing of noncoding regions occurs in disease but is often uncorrelated with ADAR levels, underscoring the need tostudy deaminase-independent control of editing. C.elegans have two ADAR proteins, ADR-2 and thetheoretically catalytically inactive ADR-1. Using high-throughput RNA sequencing of wild-type and adr mutant worms, we expand the repertoire of C.elegans edited transcripts over 5-fold and confirm that ADR-2 is the only active deaminase invivo. Despite lacking deaminase function, ADR-1 affects editing of over 60 adenosines within the 3' UTRs of 16 different mRNAs. Furthermore, ADR-1 interacts directly with ADR-2 substrates, even in the absence of ADR-2, and mutations within its double-stranded RNA (dsRNA) binding domains abolish both binding and editing regulation. We conclude that ADR-1 acts as a major regulator of editing by binding ADR-2 substrates invivo. These results raise the possibility that other dsRNA binding proteins, including the inactive human ADARs, regulate RNA editing through deaminase-independent mechanisms. Experimental Details: RNASeq.elegans.WBStrain00000439.WBls:0000002.Hermaphrodite.WBbt:0007833.SRP031831.SRX366861 RNASeq.elegans.WBStrain00000437.WBls:0000002.Hermaphrodite.WBbt:0007833.SRP031831.SRX366862 RNASeq.elegans.WBStrain00000438.WBls:0000002.Hermaphrodite.WBbt:0007833.SRP031831.SRX366863 RNASeq.elegans.WBStrain00000001.WBls:0000002.Hermaphrodite.WBbt:0007833.SRP031831.SRX366864 RNASeq.elegans.WBStrain00000439.WBls:0000002.Hermaphrodite.WBbt:0007833.SRP031831.SRX366865 RNASeq.elegans.WBStrain00000439.WBls:0000002.Hermaphrodite.WBbt:0007833.SRP031831.SRX366866 RNASeq.elegans.WBStrain00000439.WBls:0000002.Hermaphrodite.WBbt:0007833.SRP031831.SRX366867 RNASeq.elegans.WBStrain00000439.WBls:0000002.Hermaphrodite.WBbt:0007833.SRP031831.SRX366868. |
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