SPELL - Nematode

Citation	Sorokin EP, Gasch AP, Kimble J. Competence for chemical reprogramming of sexual fate correlates with an intersexual molecular signature in Caenorhabditis elegans. Genetics, 2014.
PubMed ID	25146970
Short Description	Competence for chemical reprogramming of sexual fate correlates with an intersexual molecular signature in Caenorhabditis elegans. GEO Record: GSE54030 Platform: GPL13776 Download gene-centric, log2 transformed data: WBPaper00045618.ce.rs.csv
# of Conditions	52
Full Description	In multicellular organisms, genetic programs guide cells to adopt cell fates as tissues are formed during development, maintained in adults, and repaired after injury. Here we explore how a small molecule in the environment can switch a genetic program from one fate to another. Wild-type Caenorhabditis elegans XX adult hermaphrodites make oocytes continuously, but certain mutant XX adults make sperm instead in an otherwise hermaphrodite soma. Thus, puf-8; lip-1 XX adults make only sperm, but they can be switched from sperm to oocyte production by treatment with a small-molecule MEK inhibitor. To ask whether this chemical reprogramming is common, we tested six XX sperm-only mutants, but found only one other capable of cell fate switching, fbf-1; lip-1. Therefore, reprogramming competence relies on genotype, with only certain mutants capable of responding to the MEK inhibitor with a cell fate change. To gain insight into the molecular basis of competence for chemical reprogramming, we compared polyadenylated transcriptomes of competent and noncompetent XX sperm-only mutants in the absence of the MEK inhibitor and hence in the absence of cell fate reprogramming. Despite their cellular production of sperm, competent mutants were enriched for oogenic messenger RNAs relative to mutants lacking competence for chemical reprogramming. In addition, competent mutants expressed the oocyte-specific protein RME-2, whereas those lacking competence did not. Therefore, mutants competent for reprogramming possess an intersexual molecular profile at both RNA and protein levels. We suggest that this intersexual molecular signature is diagnostic of an intermediate network state that poises the germline tissue for changing its cellular fate in response to environmental cues. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426002 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426003 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426004 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426005 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426006 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426007 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426008 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426009 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426010 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426011 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426012 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426013 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426014 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426015 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426016 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426017 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426018 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426019 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426020 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426021 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426022 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426023 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426024 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426025 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426026 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426027 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426028 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426029 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426030 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426031 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426032 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426033 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426034 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426035 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426036 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426037 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426038 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426039 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426040 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426041 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426042 RNASeq.elegans.WBStrain00022651.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426043 RNASeq.elegans.WBStrain00022618.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426044 RNASeq.elegans.WBStrain00022618.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426045 RNASeq.elegans.WBStrain00022618.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426046 RNASeq.elegans.WBStrain00022618.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426047 RNASeq.elegans.WBStrain00022618.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426048 RNASeq.elegans.WBStrain00022618.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426049 RNASeq.elegans.WBStrain00022618.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426050 RNASeq.elegans.WBStrain00022618.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426051 RNASeq.elegans.WBStrain00022618.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426052 RNASeq.elegans.WBStrain00022618.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP035359.SRX426053.
Tags	Method: RNAseq, Species: Caenorhabditis elegans, Topic: response to chemical