SPELL - Nematode

Citation	Stoeckius M, Grun D, Rajewsky N. Paternal RNA contributions in the Caenorhabditis elegans zygote. EMBO J, 2014.
PubMed ID	24894551
Short Description	Paternal RNA contributions in the Caenorhabditis elegans zygote. GEO Record: GSE57351 Platform: GPL13776 Download gene-centric, log2 transformed data: WBPaper00045359.ce.rs.csv
# of Conditions	15
Full Description	Development of the early embryo is thought to be mainly driven by maternal gene products and post-transcriptional gene regulation. Here, we used metabolic labeling to show that RNA can be transferred by sperm into the oocyte upon fertilization. To identify genes with paternal expression in the embryo, we performed crosses of males and females from divergent Caenorhabditis elegans strains. RNA sequencing of mRNAs and small RNAs in the 1-cell hybrid embryo revealed that about one hundred sixty paternal mRNAs are reproducibly expressed in the embryo and that about half of all assayed endogenous siRNAs and piRNAs are also of paternal origin. Together, our results suggest an unexplored paternal contribution to early development. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000006.Hermaphrodite.WBbt:0007833.SRP041708.SRX533796 RNASeq.elegans.WBStrain00000001.WBls:0000006.Hermaphrodite.WBbt:0007833.SRP041708.SRX533797 RNASeq.elegans.WBStrain00000001.WBls:0000006.Hermaphrodite.WBbt:0007833.SRP041708.SRX533798 RNASeq.elegans.WBStrain00000001.WBls:0000006.Hermaphrodite.WBbt:0007833.SRP041708.SRX533799 RNASeq.elegans.WBStrain00000001.WBls:0000006.Hermaphrodite.WBbt:0007833.SRP041708.SRX533800 RNASeq.elegans.WBStrain00000001.WBls:0000006.Hermaphrodite.WBbt:0007833.SRP041708.SRX533801 RNASeq.elegans.WBStrain00004602.WBls:0000041.Hermaphrodite.WBbt:0007833.SRP041708.SRX533802 RNASeq.elegans.WBStrain00004602.WBls:0000041.Hermaphrodite.WBbt:0007833.SRP041708.SRX533803 RNASeq.elegans.WBStrain00004602.WBls:0000041.Hermaphrodite.WBbt:0007833.SRP041708.SRX533804 RNASeq.elegans.WBStrain00004602.WBls:0000041.Male.WBbt:0006798.SRP041708.SRX533805 RNASeq.elegans.WBStrain00004602.WBls:0000041.Male.WBbt:0006798.SRP041708.SRX533806 RNASeq.elegans.WBStrain00000001.WBls:0000041.Hermaphrodite.WBbt:0007833.SRP041708.SRX533807 RNASeq.elegans.WBStrain00000001.WBls:0000041.Hermaphrodite.WBbt:0007833.SRP041708.SRX533808 RNASeq.elegans.WBStrain00000001.WBls:0000041.Hermaphrodite.WBbt:0007833.SRP041708.SRX533809 RNASeq.elegans.WBStrain00000001.WBls:0000041.Male.WBbt:0006798.SRP041708.SRX533810.
Tags	Method: RNAseq, Species: Caenorhabditis elegans, Topic: gene expression - general