SPELL - Nematode

Citation	Weimer S, Priebs J, Kuhlow D, Groth M, Priebe S, Mansfeld J, Merry TL, Dubuis S, Laube B, Pfeiffer AF, Schulz TJ, Guthke R, Platzer M, Zamboni N, Zarse K, Ristow M. D-Glucosamine supplementation extends life span of nematodes and of ageing mice. Nat Commun, 2014.
PubMed ID	24714520
Short Description	D-Glucosamine supplementation extends life span of nematodes and of ageing mice. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00045095.ce.rs.csv
# of Conditions	12
Full Description	D-Glucosamine (GlcN) is a freely available and commonly used dietary supplement potentially promoting cartilage health in humans, which also acts as an inhibitor of glycolysis. Here we show that GlcN, independent of the hexosamine pathway, extends Caenorhabditis elegans life span by impairing glucose metabolism that activates AMP-activated protein kinase (AMPK/AAK-2) and increases mitochondrial biogenesis. Consistent with the concept of mitohormesis, GlcN promotes increased formation of mitochondrial reactive oxygen species (ROS) culminating in increased expression of the nematodal amino acid-transporter 1 (aat-1) gene. Ameliorating mitochondrial ROS formation or impairment of aat-1-expression abolishes GlcN-mediated life span extension in an NRF2/SKN-1-dependent fashion. Unlike other calorie restriction mimetics, such as 2-deoxyglucose, GlcN extends life span of ageing C57BL/6 mice, which show an induction of mitochondrial biogenesis, lowered blood glucose levels, enhanced expression of several murine amino-acid transporters, as well as increased amino-acid catabolism. Taken together, we provide evidence that GlcN extends life span in evolutionary distinct species by mimicking a low-carbohydrate diet. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469110 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469111 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469112 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469113 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469114 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469115 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469116 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469117 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469118 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469119 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469120 RNASeq.elegans.WBStrain00000001.WBls:0000065.Hermaphrodite.WBbt:0007833.SRP037555.SRX469121.
Tags	Method: RNAseq, Species: Caenorhabditis elegans, Topic: aging, Topic: response to chemical