| Citation | Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M, Antebi A. DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation. Dev Cell, 2014. |
| PubMed ID | 24613396 |
| Short Description | DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00045017.ce.rs.csv |
| # of Conditions | 6 |
Full Description
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Developmental timing genes catalyze stem cell progression and animal maturation programs across taxa. Caenorhabditis elegans DRE-1/FBXO11 functions in an SCF E3-ubiquitin ligase complex to regulate the transition to adult programs, but its cognate proteolytic substrates are unknown. Here, we identify the conserved transcription factor BLMP-1 as a substrate of the SCF(DRE-1/FBXO11) complex. blmp-1 deletion suppressed dre-1 mutant phenotypes and exhibited developmental timing defects opposite to dre-1. blmp-1 also opposed dre-1 for other life history traits, including entry into the dauer diapause and longevity. BLMP-1 protein was strikingly elevated upon dre-1 depletion and dysregulated in a stage- and tissue-specific manner. The role of DRE-1 in regulating BLMP-1 stability is evolutionary conserved, aswe observed direct protein interaction and degradation function for worm and human counterparts. Taken together, posttranslational regulation ofBLMP-1/BLIMP-1 by DRE-1/FBXO11 coordinates C.elegans developmental timing and other life history traits, suggesting that this two-protein module mediates metazoan maturation processes. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000035.Hermaphrodite.WBbt:0007833.SRP038704.SRX474206 RNASeq.elegans.WBStrain00000001.WBls:0000035.Hermaphrodite.WBbt:0007833.SRP038704.SRX474207 RNASeq.elegans.WBStrain00000001.WBls:0000035.Hermaphrodite.WBbt:0007833.SRP038704.SRX474208 RNASeq.elegans.WBStrain00047070.WBls:0000035.Hermaphrodite.WBbt:0007833.SRP038704.SRX474209 RNASeq.elegans.WBStrain00047070.WBls:0000035.Hermaphrodite.WBbt:0007833.SRP038704.SRX474210 RNASeq.elegans.WBStrain00047070.WBls:0000035.Hermaphrodite.WBbt:0007833.SRP038704.SRX474211. |
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