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Citation Washburn MC, Kakaradov B, Sundararaman B, Wheeler E, Hoon S, Yeo GW, Hundley HA. The dsRBP and inactive editor ADR-1 utilizes dsRNA binding to regulate A-to-I RNA editing across the C. elegans transcriptome. Cell Rep, 2014.
PubMed ID 24508457
Short Description The dsRBP and inactive editor ADR-1 utilizes dsRNA binding to regulate A-to-I RNA editing across the C. elegans transcriptome.
GEO Record: N.A. Platform: N.A.
Download gene-centric, log2 transformed data: WBPaper00044827.ce.rs.csv
# of Conditions 8
Full Description 1316625150_help Inadequate adenosine-to-inosine editing of noncoding regions occurs in disease but is often uncorrelated with ADAR levels, underscoring the need tostudy deaminase-independent control of editing. C.elegans have two ADAR proteins, ADR-2 and thetheoretically catalytically inactive ADR-1. Using high-throughput RNA sequencing of wild-type and adr mutant worms, we expand the repertoire of C.elegans edited transcripts over 5-fold and confirm that ADR-2 is the only active deaminase invivo. Despite lacking deaminase function, ADR-1 affects editing of over 60 adenosines within the 3' UTRs of 16 different mRNAs. Furthermore, ADR-1 interacts directly with ADR-2 substrates, even in the absence of ADR-2, and mutations within its double-stranded RNA (dsRNA) binding domains abolish both binding and editing regulation. We conclude that ADR-1 acts as a major regulator of editing by binding ADR-2 substrates invivo. These results raise the possibility that other dsRNA binding proteins, including the inactive human ADARs, regulate RNA editing through deaminase-independent mechanisms.
Experimental Details:
Tags 1316625150_help
Method: RNAseq, Species: Caenorhabditis elegans, Topic: RNA interference