| Citation | Huang S, Ran Q, Li XM, Bao X, Zheng C, Li XD. MACSPI enables tissue-selective proteomic and interactomic analyses in multicellular organisms. Proc Natl Acad Sci U S A, 2024. |
| PubMed ID | 38753516 |
| Short Description | MACSPI enables tissue-selective proteomic and interactomic analyses in multicellular organisms. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00066799.ce.ms.csv |
| # of Conditions | 4 |
Full Description
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Multicellular organisms are composed of many tissue types that have distinct morphologies and functions, which are largely driven by specialized proteomes and interactomes. To define the proteome and interactome of a specific type of tissue in an intact animal, we developed a localized proteomics approach called Methionine Analog-based Cell-Specific Proteomics and Interactomics (MACSPI). This method uses the tissue-specific expression of an engineered methionyl-tRNA synthetase to label proteins with a bifunctional amino acid 2-amino-5-diazirinylnonynoic acid in selected cells. We applied MACSPI in Caenorhabditis elegans, a model multicellular organism, to selectively label, capture, and profile the proteomes of the body wall muscle and the nervous system, which led to the identification of tissue-specific proteins. Using the photo-cross-linker, we successfully profiled HSP90 interactors in muscles and neurons and identified tissue-specific interactors and stress-related interactors. Our study demonstrates that MACSPI can be used to profile tissue-specific proteomes and interactomes in intact multicellular organisms. Experimental Details: MassSpec_WBPaper00066799:body-wall-muscle_rep1 MassSpec_WBPaper00066799:body-wall-muscle_rep2 MassSpec_WBPaper00066799:neuron_rep1 MassSpec_WBPaper00066799:neuron_rep2. |
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