| Citation | Matia-Gonzalez AM, Laing EE, Gerber AP. Conserved mRNA-binding proteomes in eukaryotic organisms. Nat Struct Mol Biol, 2015. |
| PubMed ID | 26595419 |
| Short Description | Conserved mRNA-binding proteomes in eukaryotic organisms. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00048910.ce.ms.csv |
| # of Conditions | 9 |
Full Description
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RNA-binding proteins (RBPs) are essential for post-transcriptional regulation of gene expression. Recent high-throughput screens have dramatically increased the number of experimentally identified RBPs; however, comprehensive identification of RBPs within living organisms is elusive. Here we describe the repertoire of 765 and 594 proteins that reproducibly interact with polyadenylated mRNAs in Saccharomyces cerevisiae and Caenorhabditis elegans, respectively. Furthermore, we report the differential association of mRNA-binding proteins (mRPBs) upon induction of apoptosis in C. elegans L4-stage larvae. Strikingly, most proteins composing mRBPomes, including components of early metabolic pathways and the proteasome, are evolutionarily conserved between yeast and C. elegans. We speculate, on the basis of our evidence that glycolytic enzymes bind distinct glycolytic mRNAs, that enzyme-mRNA interactions relate to an ancient mechanism for post-transcriptional coordination of metabolic pathways that perhaps was established during the transition from the early 'RNA world' to the 'protein world'. Experimental Details: MassSpec_WBPaper00048910:Mixed-stage_rep1 MassSpec_WBPaper00048910:Mixed-stage_rep2 MassSpec_WBPaper00048910:Mixed-stage_rep3 MassSpec_WBPaper00048910:L4_rep1 MassSpec_WBPaper00048910:L4_rep2 MassSpec_WBPaper00048910:L4_rep3 MassSpec_WBPaper00048910:L4-ENU_rep1 MassSpec_WBPaper00048910:L4-ENU_rep2 MassSpec_WBPaper00048910:L4-ENU_rep3. |
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