| Citation | Stadler M, Artiles K, Pak J, Fire A. Contributions of mRNA abundance, ribosome loading, and post- or peri-translational effects to temporal repression of C. elegans heterochronic miRNA targets. Genome Res, 2012. |
| PubMed ID | 22855835 |
| Short Description | Contributions of mRNA abundance, ribosome loading, and post- or peri-translational effects to temporal repression of C. elegans heterochronic miRNA targets. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00041361.ce.rs.csv |
| # of Conditions | 17 |
Full Description
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miRNAs are post-transcriptional regulators of gene activity that reduce protein accumulation from target mRNAs. Elucidating precise molecular effects that animal miRNAs have on target transcripts has proven complex, with varied evidence indicating that miRNA regulation may produce different molecular outcomes in different species, systems, and/or physiological conditions. Here we use high-throughput ribosome profiling to analyze detailed translational parameters for five well-studied targets of miRNAs that regulate C. elegans developmental timing. For two targets of the miRNA lin-4 (lin-14 and lin-28), functional down-regulation was associated with decreases in both overall mRNA abundance and ribosome loading; however, these changes were of substantially smaller magnitude than corresponding changes observed in protein abundance. For three functional targets of the let-7 miRNA family for which down-regulation is critical in temporal progression of the animal (daf-12, hbl-1, and lin-41), we observed only modest changes in mRNA abundance and ribosome loading. lin-41 provides a striking example in that populations of ribosome-protected fragments from this gene remained essentially unchanged during the L3-L4 time interval when lin-41 activity is substantially down-regulated by let-7. Spectra of ribosomal positions were also examined for the five lin-4 and let-7 target mRNAs as a function of developmental time, with no indication of miRNA-induced ribosomal drop-off or significant pauses in translation. These data are consistent with models in which physiological regulation by this set of C. elegans miRNAs derives from combinatorial effects including suppressed recruitment/activation of translational machinery, compromised stability of target messages, and post- or peri-translational effects on lifetimes of polypeptide products. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000024.Hermaphrodite.WBbt:0007833.SRP014427.SRX160147 RNASeq.elegans.WBStrain00000001.WBls:0000024.Hermaphrodite.WBbt:0007833.SRP014427.SRX160148 RNASeq.elegans.WBStrain00000001.WBls:0000024.Hermaphrodite.WBbt:0007833.SRP014427.SRX160149 RNASeq.elegans.WBStrain00000001.WBls:0000024.Hermaphrodite.WBbt:0007833.SRP014427.SRX160157 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP014427.SRX160290 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP014427.SRX160291 RNASeq.elegans.WBStrain00000001.WBls:0000024.Hermaphrodite.WBbt:0007833.SRP014427.SRX160292 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP014427.SRX160293 RNASeq.elegans.WBStrain00000001.WBls:0000027.Hermaphrodite.WBbt:0007833.SRP014427.SRX160510 RNASeq.elegans.WBStrain00000001.WBls:0000027.Hermaphrodite.WBbt:0007833.SRP014427.SRX160511 RNASeq.elegans.WBStrain00000001.WBls:0000027.Hermaphrodite.WBbt:0007833.SRP014427.SRX160512 RNASeq.elegans.WBStrain00000001.WBls:0000027.Hermaphrodite.WBbt:0007833.SRP014427.SRX160513 RNASeq.elegans.WBStrain00000001.WBls:0000024.Hermaphrodite.WBbt:0007833.SRP014427.SRX160514 RNASeq.elegans.WBStrain00000001.WBls:0000024.Hermaphrodite.WBbt:0007833.SRP014427.SRX160515 RNASeq.elegans.WBStrain00000001.WBls:0000024.Hermaphrodite.WBbt:0007833.SRP014427.SRX160516 RNASeq.elegans.WBStrain00000001.WBls:0000024.Hermaphrodite.WBbt:0007833.SRP014427.SRX160517 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP014427.SRX160518. |
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