| Citation | Depuydt G, Xie F, Petyuk VA, Shanmugam N, Smolders A, Dhondt I, Brewer HM, Camp DG, Smith RD, Braeckman BP. Reduced insulin/insulin-like growth factor-1 signaling and dietary restriction inhibit translation but preserve muscle mass in Caenorhabditis elegans. Mol Cell Proteomics, 2013. |
| PubMed ID | 24002365 |
| Short Description | Reduced insulin/insulin-like growth factor-1 signaling and dietary restriction inhibit translation but preserve muscle mass in Caenorhabditis elegans. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00044128.ce.ms.csv |
| # of Conditions | 15 |
Full Description
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Reduced signaling through the C. elegans insulin/insulin-like growth factor-1-like tyrosine kinase receptor daf-2 and dietary restriction via bacterial dilution are two well-characterized lifespan-extending interventions that operate in parallel or through (partially) independent mechanisms. Using accurate mass and time tag LC-MS/MS quantitative proteomics, we detected that the abundance of a large number of ribosomal subunits is decreased in response to dietary restriction, as well as in the daf-2(e1370) insulin/insulin-like growth factor-1-receptor mutant. In addition, general protein synthesis levels in these long-lived worms are repressed. Surprisingly, ribosomal transcript levels were not correlated to actual protein abundance, suggesting that post-transcriptional regulation determines ribosome content. Proteomics also revealed the increased presence of many structural muscle cell components in long-lived worms, which appeared to result from the prioritized preservation of muscle cell volume in nutrient-poor conditions or low insulin-like signaling. Activation of DAF-16, but not diet restriction, stimulates mRNA expression of muscle-related genes to prevent muscle atrophy. Important daf-2-specific proteome changes include overexpression of aerobic metabolism enzymes and general activation of stress-responsive and immune defense systems, whereas the increased abundance of many protein subunits of the proteasome core complex is a dietary-restriction-specific characteristic. Experimental Details: MassSpec_WBPaper00044128:control-FullyFed_rep1 MassSpec_WBPaper00044128:control-FullyFed_rep2 MassSpec_WBPaper00044128:control-FullyFed_rep3 MassSpec_WBPaper00044128:control-FullyFed_rep4 MassSpec_WBPaper00044128:control-FullyFed_rep5 MassSpec_WBPaper00044128:control-DietaryRestricted_rep1 MassSpec_WBPaper00044128:control-DietaryRestricted_rep2 MassSpec_WBPaper00044128:control-DietaryRestricted_rep3 MassSpec_WBPaper00044128:control-DietaryRestricted_rep4 MassSpec_WBPaper00044128:control-DietaryRestricted_rep5 MassSpec_WBPaper00044128:daf2-FullyFed_rep1 MassSpec_WBPaper00044128:daf2-FullyFed_rep2 MassSpec_WBPaper00044128:daf2-FullyFed_rep3 MassSpec_WBPaper00044128:daf2-FullyFed_rep4 MassSpec_WBPaper00044128:daf2-FullyFed_rep5. |
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