SPELL - Nematode

Citation	Harvald EB, Sprenger RR, Dall KB, Ejsing CS, Nielsen R, Mandrup S, Murillo AB, Larance M, Gartner A, Lamond AI, Faergeman NJ. Multi-omics Analyses of Starvation Responses Reveal a Central Role for Lipoprotein Metabolism in Acute Starvation Survival in C.elegans. Cell Syst, 2017.
PubMed ID	28734827
Short Description	Multi-omics Analyses of Starvation Responses Reveal a Central Role for Lipoprotein Metabolism in Acute Starvation Survival in C.elegans. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00051532.ce.ms.csv
# of Conditions	42
Full Description	Starvation causes comprehensive metabolic changes, which are still not fully understood. Here, we used quantitative proteomics and RNA sequencing to examine the temporal starvation responses in wild-type Caenorhabditis elegans and animals lacking the transcription factor HLH-30. Our findings show that starvation alters the abundance of hundreds of proteins and mRNAs in a temporal manner, many of which are involved in central metabolic pathways, including lipoprotein metabolism. We demonstrate that premature death of hlh-30 animals under starvation can be prevented by knockdown of either vit-1 or vit-5, encoding two different lipoproteins. We further show that the size and number of intestinal lipid droplets under starvation are altered in hlh-30 animals, which can be rescued by knockdown of vit-1. Taken together, this indicates that survival of hlh-30 animals under starvation is closely linked to regulation of intestinal lipid stores. We provide the most detailed poly-omic analysis of starvation responses to date, which serves as a resource for further mechanistic studies of starvation. Experimental Details: MassSpec_WBPaper00051532:N2_fed_RepA MassSpec_WBPaper00051532:N2_fed_RepB MassSpec_WBPaper00051532:N2_fed_RepC MassSpec_WBPaper00051532:N2_1hr-starvation_RepA MassSpec_WBPaper00051532:N2_1hr-starvation_RepB MassSpec_WBPaper00051532:N2_1hr-starvation_RepC MassSpec_WBPaper00051532:N2_2hr-starvation_RepA MassSpec_WBPaper00051532:N2_2hr-starvation_RepB MassSpec_WBPaper00051532:N2_2hr-starvation_RepC MassSpec_WBPaper00051532:N2_3hr-starvation_RepA MassSpec_WBPaper00051532:N2_3hr-starvation_RepB MassSpec_WBPaper00051532:N2_3hr-starvation_RepC MassSpec_WBPaper00051532:N2_4hr-starvation_RepA MassSpec_WBPaper00051532:N2_4hr-starvation_RepB MassSpec_WBPaper00051532:N2_4hr-starvation_RepC MassSpec_WBPaper00051532:N2_6hr-starvation_RepA MassSpec_WBPaper00051532:N2_6hr-starvation_RepB MassSpec_WBPaper00051532:N2_6hr-starvation_RepC MassSpec_WBPaper00051532:N2_16hr-starvation_RepA MassSpec_WBPaper00051532:N2_16hr-starvation_RepB MassSpec_WBPaper00051532:N2_16hr-starvation_RepC MassSpec_WBPaper00051532:hlh-30(tm1978)_fed_RepA MassSpec_WBPaper00051532:hlh-30(tm1978)_fed_RepB MassSpec_WBPaper00051532:hlh-30(tm1978)_fed_RepC MassSpec_WBPaper00051532:hlh-30(tm1978)_1hr-starvation_RepA MassSpec_WBPaper00051532:hlh-30(tm1978)_1hr-starvation_RepB MassSpec_WBPaper00051532:hlh-30(tm1978)_1hr-starvation_RepC MassSpec_WBPaper00051532:hlh-30(tm1978)_2hr-starvation_RepA MassSpec_WBPaper00051532:hlh-30(tm1978)_2hr-starvation_RepB MassSpec_WBPaper00051532:hlh-30(tm1978)_2hr-starvation_RepC MassSpec_WBPaper00051532:hlh-30(tm1978)_3hr-starvation_RepA MassSpec_WBPaper00051532:hlh-30(tm1978)_3hr-starvation_RepB MassSpec_WBPaper00051532:hlh-30(tm1978)_3hr-starvation_RepC MassSpec_WBPaper00051532:hlh-30(tm1978)_4hr-starvation_RepA MassSpec_WBPaper00051532:hlh-30(tm1978)_4hr-starvation_RepB MassSpec_WBPaper00051532:hlh-30(tm1978)_4hr-starvation_RepC MassSpec_WBPaper00051532:hlh-30(tm1978)_6hr-starvation_RepA MassSpec_WBPaper00051532:hlh-30(tm1978)_6hr-starvation_RepB MassSpec_WBPaper00051532:hlh-30(tm1978)_6hr-starvation_RepC MassSpec_WBPaper00051532:hlh-30(tm1978)_16hr-starvation_RepA MassSpec_WBPaper00051532:hlh-30(tm1978)_16hr-starvation_RepB MassSpec_WBPaper00051532:hlh-30(tm1978)_16hr-starvation_RepC.
Tags	Method: proteomics, Species: Caenorhabditis elegans, Topic: lipid metabolic process