|Citation||Conine CC, Moresco JJ, Gu W, Shirayama M, Conte D, Yates JR, Mello CC. Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans. Cell, 2013.|
|Short Description||Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans. |
GEO Record: GSE49672 Platform: GPL13776
Download gene-centric, log2 transformed data: WBPaper00044616.ce.rs.csv
|# of Conditions||4|
|Full Description||During each life cycle, germ cells preserve and pass on both genetic and epigenetic information. In C.elegans, the ALG-3/4 Argonaute proteins are expressed during male gametogenesis and promote male fertility. Here, we show that the CSR-1 Argonaute functions with ALG-3/4 to positively regulate target genes required for spermiogenesis. Our findings suggest that ALG-3/4 functions during spermatogenesis to amplify a small RNA signal that represents an epigenetic memory of male-specific gene expression. CSR-1, which is abundant in mature sperm, appears to transmit this memory to offspring. Surprisingly, in addition to small RNAs targeting male-specific genes, we show that males also harbor an extensive repertoire of CSR-1 smallRNAs targeting oogenesis-specific mRNAs. Together, these findings suggest that C.elegans sperm transmit not only the genome but also epigenetic binary signals in the form of Argonaute/small RNA complexes that constitute a memory of gene expression in preceding generations.