SPELL - Nematode

Citation	McColl G, Rogers AN, Alavez S, Hubbard AE, Melov S, Link CD, Bush AI, Kapahi P, Lithgow GJ. Insulin-like signaling determines survival during stress via posttranscriptional mechanisms in C. elegans. Cell Metab, 2010.
PubMed ID	20816092
Short Description	Insulin-like signaling determines survival during stress via posttranscriptional mechanisms in C. elegans. GEO Record: GSE22383 Platform: GPL9458 Download gene-centric, log2 transformed data: WBPaper00037147.ce.mr.csv
# of Conditions	72
Full Description	The insulin-like signaling (ILS) pathway regulates metabolism and is known to modulate adult life span in C. elegans. Altered stress responses and resistance to a wide range of stressors are also associated with changes in ILS and contribute to enhanced longevity. The transcription factors DAF-16 and HSF-1 are key effectors of the longevity phenotype. We demonstrate that increased intrinsic thermotolerance, due to lower ILS, is not dependent on stress-induced transcriptional responses but instead requires active protein translation. Translation profiling experiments reveal genes that are posttranscriptionally regulated in response to altered ILS during heat shock in a DAF-16-dependent manner. Furthermore, several novel proteins are specifically required for ILS effects on thermotolerance. We propose that lowered ILS results in metabolic and physiological changes. These DAF-16-induced changes precondition a translational response under acute stress to modulate survival. Experimental Details: WBPaper00037147:N2_control_Fraction1_group1 WBPaper00037147:N2_control_Fraction2_group1 WBPaper00037147:N2_control_Fraction3_group1 WBPaper00037147:N2_HeatShocked_Fraction1_group1 WBPaper00037147:N2_HeatShocked_Fraction2_group1 WBPaper00037147:N2_HeatShocked_Fraction3_group1 WBPaper00037147:N2_control_Fraction1_group2 WBPaper00037147:N2_control_Fraction2_group2 WBPaper00037147:N2_control_Fraction3_group2 WBPaper00037147:N2_HeatShocked_Fraction1_group2 WBPaper00037147:N2_HeatShocked_Fraction2_group2 WBPaper00037147:N2_HeatShocked_Fraction3_group2 WBPaper00037147:N2_control_Fraction1_group3 WBPaper00037147:N2_control_Fraction2_group3 WBPaper00037147:N2_control_Fraction3_group3 WBPaper00037147:N2_HeatShocked_Fraction1_group3 WBPaper00037147:N2_HeatShocked_Fraction2_group3 WBPaper00037147:N2_HeatShocked_Fraction3_group3 WBPaper00037147:N2_control_Fraction1_group4 WBPaper00037147:N2_control_Fraction2_group4 WBPaper00037147:N2_control_Fraction3_group4 WBPaper00037147:N2_HeatShocked_Fraction1_group4 WBPaper00037147:N2_HeatShocked_Fraction2_group4 WBPaper00037147:N2_HeatShocked_Fraction3_group4 WBPaper00037147:daf-2(e1370)_control_Fraction1_group1 WBPaper00037147:daf-2(e1370)_control_Fraction2_group1 WBPaper00037147:daf-2(e1370)_control_Fraction3_group1 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction1_group1 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction2_group1 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction3_group1 WBPaper00037147:daf-2(e1370)_control_Fraction1_group2 WBPaper00037147:daf-2(e1370)_control_Fraction2_group2 WBPaper00037147:daf-2(e1370)_control_Fraction3_group2 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction1_group2 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction2_group2 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction3_group2 WBPaper00037147:daf-2(e1370)_control_Fraction1_group3 WBPaper00037147:daf-2(e1370)_control_Fraction2_group3 WBPaper00037147:daf-2(e1370)_control_Fraction3_group3 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction1_group3 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction2_group3 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction3_group3 WBPaper00037147:daf-2(e1370)_control_Fraction1_group4 WBPaper00037147:daf-2(e1370)_control_Fraction2_group4 WBPaper00037147:daf-2(e1370)_control_Fraction3_group4 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction1_group4 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction2_group4 WBPaper00037147:daf-2(e1370)_HeatShocked_Fraction3_group4 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction1_group1 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction2_group1 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction3_group1 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction1_group1 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction2_group1 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction3_group1 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction1_group2 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction2_group2 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction3_group2 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction1_group2 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction2_group2 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction3_group2 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction1_group3 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction2_group3 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction3_group3 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction1_group3 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction2_group3 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction3_group3 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction1_group4 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction2_group4 WBPaper00037147:daf16(M26);daf-2(e1370)_control_Fraction3_group4 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction1_group4 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction2_group4 WBPaper00037147:daf16(M26);daf-2(e1370)_HeatShocked_Fraction3_group4.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: response to temperature stimulus