SPELL - Nematode

Citation	Huumonen K, Immonen HK, Baverstock K, Hiltunen M, Korkalainen M, Lahtinen T, Parviainen J, Viluksela M, Wong G, Naarala J, Juutilainen J. Radiation-induced genomic instability in Caenorhabditis elegans. Mutat Res, 2012.
PubMed ID	22796420
Short Description	Radiation-induced genomic instability in Caenorhabditis elegans. GEO Record: GSE29979 Platform: GPL9458 Download gene-centric, log2 transformed data: WBPaper00041300.ce.mr.csv
# of Conditions	8
Full Description	Radiation-induced genomic instability has been well documented, particularly in vitro. However, the understanding of its mechanisms and their consequences in vivo is still limited. In this study, Caenorhabditis elegans (C. elegans; strain CB665) nematodes were exposed to X-rays at doses of 0.1, 1, 3 or 10Gy. The endpoints were measured several generations after exposure and included mutations in the movement-related gene unc-58, alterations in gene expression analysed with oligoarrays containing the entire C. elegans genome, and micro-satellite mutations measured by capillary electrophoresis. The progeny of the irradiated nematodes showed an increased mutation frequency in the unc-58 gene, with a maximum response observed at 1Gy. Significant differences were also found in gene expression between the irradiated (1Gy) and non-irradiated nematode lines. Differences in gene expression did not show clear clustering into certain gene categories, suggesting that the instability might be a chaotic process rather than a result of changes in the function of few specific genes such as, e.g., those responsible for DNA repair. Increased heterogeneity in gene expression, which has previously been described in irradiated cultured human lymphocytes, was also observed in the present study in C. elegans, the coefficient of variation of gene expression being higher in the progeny of irradiated nematodes than in control nematodes. To the best of our knowledge, this is the first publication reporting radiation-induced genomic instability in C. elegans. Experimental Details: WBPaper00041300:Control_rep1 WBPaper00041300:Control_rep7 WBPaper00041300:Control_rep9 WBPaper00041300:Control_rep10 WBPaper00041300:X_radiation_1_Gy_rep2 WBPaper00041300:X_radiation_1_Gy_rep3 WBPaper00041300:X_radiation_1_Gy_rep5 WBPaper00041300:X_radiation_1_Gy_rep15.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: response to radiation