SPELL - Nematode

Citation	Twumasi-Boateng K, Shapira M. Dissociation of immune responses from pathogen colonization supports pattern recognition in C. elegans. PLoS One, 2012.
PubMed ID	22514739
Short Description	Dissociation of immune responses from pathogen colonization supports pattern recognition in C. elegans. GEO Record: GSE39536 Platform: GPL9458 Download gene-centric, log2 transformed data: WBPaper00040990.ce.mr.csv
# of Conditions	9
Full Description	Caenorhabditis elegans has been used for over a decade to characterize signaling cascades controlling innate immune responses. However, what initiates these responses in the worm has remained elusive. To gain a better understanding of the initiating events we delineated genome-wide immune responses to the bacterial pathogen Pseudomonas aeruginosa in worms heavily-colonized by the pathogen versus worms visibly not colonized. We found that infection responses in both groups were identical, suggesting that immune responses were not correlated with colonization and its associated damage. Quantitative RT-PCR measurements further showed that pathogen secreted factors were not able to induce an immune response, but exposure to a non-pathogenic Pseudomonas species was. These findings raise the possibility that the C.elegans immune response is initiated by recognition of microbe-associated molecular patterns. In the absence of orthologs of known pattern recognition receptors, C. elegans may rely on novel mechanisms, thus holding the potential to advance our understanding of evolutionarily conserved strategies for pathogen recognition. Experimental Details: WBPaper00040990:OP50_HandPicked_rep1 WBPaper00040990:OP50_WormSorter_rep2 WBPaper00040990:OP50_WormSorter_rep3 WBPaper00040990:PA14_non-colonized_HandPicked_rep1 WBPaper00040990:PA14_non-colonized_WormSorter_rep2 WBPaper00040990:PA14_non-colonized_WormSorter_rep3 WBPaper00040990:PA14_colonized_HandPicked_rep1 WBPaper00040990:PA14_colonized_WormSorter_rep2 WBPaper00040990:PA14_colonized_WormSorter_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: innate immune response