| Citation | Petrella LN, Wang W, Spike CA, Rechtsteiner A, Reinke V, Strome S. synMuv B proteins antagonize germline fate in the intestine and ensure C. elegans survival. Development, 2011. |
| PubMed ID | 21343362 |
| Short Description | synMuv B proteins antagonize germline fate in the intestine and ensure C. elegans survival. GEO Record: GSE26825 GSE26824 GSE26823 Platform: GPL5859 Download gene-centric, log2 transformed data: WBPaper00038168.ce.mr.csv |
| # of Conditions | 12 |
Full Description
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Previous studies demonstrated that a subset of synMuv B mutants ectopically misexpress germline-specific P-granule proteins in their somatic cells, suggesting a failure to properly orchestrate a soma/germline fate decision. Surprisingly, this fate confusion does not affect viability at low to ambient temperatures. Here, we show that, when grown at high temperature, a majority of synMuv B mutants irreversibly arrest at the L1 stage. High temperature arrest (HTA) is accompanied by upregulation of many genes characteristic of germ line, including genes encoding components of the synaptonemal complex and other meiosis proteins. HTA is suppressed by loss of global regulators of germline chromatin, including MES-4, MRG-1, ISW-1 and the MES-2/3/6 complex, revealing that arrest is caused by somatic cells possessing a germline-like chromatin state. Germline genes are preferentially misregulated in the intestine, and necessity and sufficiency tests demonstrate that the intestine is the tissue responsible for HTA. We propose that synMuv B mutants fail to erase or antagonize an inherited germline chromatin state in somatic cells during embryonic and early larval development. As a consequence, somatic cells gain a germline program of gene expression in addition to their somatic program, leading to a mixed fate. Somatic expression of germline genes is enhanced at elevated temperature, leading to developmentally compromised somatic cells and arrest of newly hatched larvae. Experimental Details: WBPaper00038168:lin-15B_rep1 WBPaper00038168:lin-15B_rep2 WBPaper00038168:lin-15B_rep3 WBPaper00038168:lin-15B_rep4 WBPaper00038168:lin-35_rep1 WBPaper00038168:lin-35_rep2 WBPaper00038168:lin-35_rep3 WBPaper00038168:lin-35_rep4 WBPaper00038168:lin-35_mes-4(RNAi)_rep1 WBPaper00038168:lin-35_mes-4(RNAi)_rep2 WBPaper00038168:lin-35_mes-4(RNAi)_rep3 WBPaper00038168:lin-35_mes-4(RNAi)_rep4. |
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