| Citation | Vinuela A, Snoek LB, Riksen JA, Kammenga JE. Genome-wide gene expression regulation as a function of genotype and age in C. elegans. Genome Res, 2010. |
| PubMed ID | 20488933 |
| Short Description | Genome-wide gene expression regulation as a function of genotype and age in C. elegans. GEO Record: GSE17071 Platform: GPL4038 Download gene-centric, log2 transformed data: WBPaper00036286.ce.mr.csv |
| # of Conditions | 54 |
Full Description
|
Gene expression becomes more variable with age, and it is widely assumed that this is due to a decrease in expression regulation. But currently there is no understanding how gene expression regulatory patterns progress with age. Here we explored genome-wide gene expression variation and regulatory loci (eQTL) in a population of developing and aging C. elegans recombinant inbred worms. We found almost 900 genes with an eQTL, of which almost half were found to have a genotype-by-age effect ((gxa)eQTL). The total number of eQTL decreased with age, whereas the variation in expression increased. In developing worms, the number of genes with increased expression variation (1282) was similar to the ones with decreased expression variation (1328). In aging worms, the number of genes with increased variation (1772) was nearly five times higher than the number of genes with a decreased expression variation (373). The number of cis-acting eQTL in juveniles decreased by almost 50% in old worms, whereas the number of trans-acting loci decreased by approximately 27%, indicating that cis-regulation becomes relatively less frequent than trans-regulation in aging worms. Of the 373 genes with decreased expression level variation in aging worms, approximately 39% had an eQTL compared with approximately 14% in developing worms. (gxa)eQTL were found for approximately 21% of these genes in aging worms compared with only approximately 6% in developing worms. We highlight three examples of linkages: in young worms (pgp-6), in old worms (daf-16), and throughout life (lips-16). Our findings demonstrate that eQTL patterns are strongly affected by age, and suggest that gene network integrity declines with age. Experimental Details: WBPaper00036286:WN8_vs_WN41_T1 WBPaper00036286:WN60_vs_WN77_T1 WBPaper00036286:WN9_vs_WN62_T1 WBPaper00036286:WN71_vs_WN79_T1 WBPaper00036286:WN27_vs_WN56_T1 WBPaper00036286:WN46_vs_WN69_T1 WBPaper00036286:WN68_vs_WN37_T1 WBPaper00036286:WN13_vs_WN21_T1 WBPaper00036286:WN20_vs_WN53_T1 WBPaper00036286:WN57_vs_WN32_T1 WBPaper00036286:WN19_vs_WN39_T1 WBPaper00036286:WN76_vs_WN59_T1 WBPaper00036286:WN28_vs_WN50_T1 WBPaper00036286:WN44_vs_WN2_T1 WBPaper00036286:WN24_vs_WN16_T1 WBPaper00036286:WN11_vs_WN25_T1 WBPaper00036286:WN19_vs_WN1_T1 WBPaper00036286:WN33_vs_WN3_T1 WBPaper00036286:WN8_vs_WN41_T2 WBPaper00036286:WN60_vs_WN77_T2 WBPaper00036286:WN9_vs_WN62_T2 WBPaper00036286:WN71_vs_WN79_T2 WBPaper00036286:WN27_vs_WN56_T2 WBPaper00036286:WN46_vs_WN69_T2 WBPaper00036286:WN68_vs_WN37_T2 WBPaper00036286:WN13_vs_WN21_T2 WBPaper00036286:WN20_vs_WN53_T2 WBPaper00036286:WN57_vs_WN32_T2 WBPaper00036286:WN19_vs_WN39_T2 WBPaper00036286:WN76_vs_WN59_T2 WBPaper00036286:WN28_vs_WN50_T2 WBPaper00036286:WN44_vs_WN2_T2 WBPaper00036286:WN24_vs_WN16_T2 WBPaper00036286:WN11_vs_WN25_T2 WBPaper00036286:WN19_vs_WN1_T2 WBPaper00036286:WN33_vs_WN3_T2 WBPaper00036286:WN8_vs_WN41_T3 WBPaper00036286:WN60_vs_WN77_T3 WBPaper00036286:WN9_vs_WN62_T3 WBPaper00036286:WN71_vs_WN79_T3 WBPaper00036286:WN27_vs_WN56_T3 WBPaper00036286:WN46_vs_WN69_T3 WBPaper00036286:WN68_vs_WN37_T3 WBPaper00036286:WN13_vs_WN21_T3 WBPaper00036286:WN20_vs_WN53_T3 WBPaper00036286:WN57_vs_WN32_T3 WBPaper00036286:WN19_vs_WN39_T3 WBPaper00036286:WN76_vs_WN59_T3 WBPaper00036286:WN28_vs_WN50_T3 WBPaper00036286:WN44_vs_WN2_T3 WBPaper00036286:WN24_vs_WN16_T3 WBPaper00036286:WN11_vs_WN25_T3 WBPaper00036286:WN19_vs_WN1_T3 WBPaper00036286:WN33_vs_WN3_T3. |
|
Tags
|
Contact: help (at) wormbase.org
