| Citation | Leacock SW, Reinke V. Expression profiling of MAP kinase-mediated meiotic progression in Caenorhabditis elegans. PLoS Genet, 2006. |
| PubMed ID | 17096596 |
| Short Description | Expression profiling of MAP kinase-mediated meiotic progression in Caenorhabditis elegans. GEO Record: GSE5069 Platform: GPL446 GPL539 GPL540 GPL3859 GPL3860 GPL3861 Download gene-centric, log2 transformed data: WBPaper00028788.ce.mr.csv |
| # of Conditions | 24 |
Full Description
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The LET-60 (Ras)/LIN-45 (Raf)/MPK-1 (MAP kinase) signaling pathway plays a key role in the development of multiple tissues in Caenorhabditis elegans. For the most part, the identities of the downstream genes that act as the ultimate effectors of MPK-1 signaling have remained elusive. A unique allele of mpk-1, ga111, displays a reversible, temperature-sensitive, tissue-specific defect in progression through meiotic prophase I. We performed gene expression profiling on mpk-1(ga111) animals to identify candidate downstream effectors of MPK-1 signaling in the germ line. This analysis delineated a cohort of genes whose expression requires MPK-1 signaling in germ cells in the pachytene stage of meiosis I. RNA in situ hybridization analysis shows that these genes are expressed in the germ line in an MPK-1-dependent manner and have a spatial expression pattern consistent with the location of activated MPK-1. We found that one MPK-1 signaling-responsive gene encoding a C2H2 zinc finger protein plays a role in meiotic chromosome segregation downstream of MPK-1. Additionally, discovery of genes responsive to MPK-1 signaling permitted us to order MPK-1 signaling relative to several events occurring in pachytene, including EFL-1/DPL-1 gene regulation and X chromosome reactivation. This study highlights the utility of applying global gene expression methods to investigate genes downstream of commonly used signaling pathways in vivo. Experimental Details: WBPaper00028788:0_hr_unc-79_fem-1_vs_ref_1 WBPaper00028788:0_hr_unc-79_fem-1_vs_ref_2 WBPaper00028788:0_hr_unc-79_fem-1_vs_ref_3 WBPaper00028788:0_hr_unc-79_mpk-1_fem-1_vs_ref_1 WBPaper00028788:0_hr_unc-79_mpk-1_fem-1_vs_ref_2 WBPaper00028788:0_hr_unc-79_mpk-1_fem-1_vs_ref_3 WBPaper00028788:3_hr_unc-79_mpk-1_fem-1_vs_ref_1 WBPaper00028788:3_hr_unc-79_mpk-1_fem-1_vs_ref_2 WBPaper00028788:3_hr_unc-79_mpk-1_fem-1_vs_ref_3 WBPaper00028788:6_hr_unc-79_mpk-1_fem-1_vs_ref_1 WBPaper00028788:6_hr_unc-79_mpk-1_fem-1_vs_ref_2 WBPaper00028788:6_hr_unc-79_mpk-1_fem-1_vs_ref_3 WBPaper00028788:9_hr_unc-79_mpk-1_fem-1_vs_ref_1 WBPaper00028788:9_hr_unc-79_mpk-1_fem-1_vs_ref_2 WBPaper00028788:12_hr_unc-79_mpk-1_fem-1_vs_ref_1 WBPaper00028788:12_hr_unc-79_mpk-1_fem-1_vs_ref_2 WBPaper00028788:12_hr_unc-79_mpk-1_fem-1_vs_ref_3 WBPaper00028788:15_hr_unc-79_mpk-1_fem-1_vs_ref_1 WBPaper00028788:15_hr_unc-79_mpk-1_fem-1_vs_ref_2 WBPaper00028788:15_hr_unc-79_mpk-1_fem-1_vs_ref_3 WBPaper00028788:15_hr_unc-79_fem-1_vs_ref_1 WBPaper00028788:15_hr_unc-79_fem-1_vs_ref_2 WBPaper00028788:15_hr_unc-79_fem-1_vs_ref_3 WBPaper00028788:9_hr_unc-79_mpk-1_fem-1_vs_ref_3. |
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