| Citation | Zhang L, Hammell M, Kudlow BA, Ambros V, Han M. Systematic analysis of dynamic miRNA-target interactions during C. elegans development. Development, 2009. |
| PubMed ID | 19675127 |
| Short Description | Systematic analysis of dynamic miRNA-target interactions during C. elegans development. GEO Record: GSE16208 Platform: GPL5883 Download gene-centric, log2 transformed data: WBPaper00035084.ce.mr.csv |
| # of Conditions | 36 |
Full Description
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Although microRNA (miRNA)-mediated functions have been implicated in many aspects of animal development, the majority of miRNA::mRNA regulatory interactions remain to be characterized experimentally. We used an AIN/GW182 protein immunoprecipitation approach to systematically analyze miRNA::mRNA interactions during C. elegans development. We characterized the composition of miRNAs in functional miRNA-induced silencing complexes (miRISCs) at each developmental stage and identified three sets of miRNAs with distinct stage-specificity of function. We then identified thousands of miRNA targets in each developmental stage, including a significant portion that is subject to differential miRNA regulation during development. By identifying thousands of miRNA family-mRNA pairs with temporally correlated patterns of AIN-2 association, we gained valuable information on the principles of physiological miRNA::target recognition and predicted 1589 high-confidence miRNA family::mRNA interactions. Our data support the idea that miRNAs preferentially target genes involved in signaling processes and avoid genes with housekeeping functions, and that miRNAs orchestrate temporal developmental programs by coordinately targeting or avoiding genes involved in particular biological functions. Experimental Details: WBPaper00035084:1_Egg_IP WBPaper00035084:1_Egg_tot WBPaper00035084:2_Egg_IP WBPaper00035084:2_Egg_tot WBPaper00035084:4_Egg_IP WBPaper00035084:4_Egg_tot WBPaper00035084:5_Egg_IP WBPaper00035084:5_Egg_tot WBPaper00035084:2_L1_IP WBPaper00035084:2_L1_tot WBPaper00035084:5_L1_IP WBPaper00035084:5_L1_tot WBPaper00035084:10_L1_IP WBPaper00035084:10_L1_tot WBPaper00035084:11_L1_IP WBPaper00035084:11_L1_tot WBPaper00035084:1_L2_IP WBPaper00035084:1_L2_tot WBPaper00035084:2_L2_IP WBPaper00035084:2_L2_tot WBPaper00035084:3_L2_IP WBPaper00035084:3_L2_tot WBPaper00035084:1_L3_IP WBPaper00035084:1_L3_tot WBPaper00035084:2_L3_IP WBPaper00035084:2_L3_tot WBPaper00035084:3_L3_IP WBPaper00035084:3_L3_tot WBPaper00035084:5_L3_IP WBPaper00035084:5_L3_tot WBPaper00035084:1_L4_IP WBPaper00035084:1_L4_tot WBPaper00035084:3_L4_IP WBPaper00035084:3_L4_tot WBPaper00035084:10_L4_IP WBPaper00035084:10_L4_tot. |
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