| Citation | Kwon JY, Hong M, Choi MS, Kang S, Duke K, Kim S, Lee S, Lee J. Ethanol-response genes and their regulation analyzed by a microarray and comparative genomic approach in the nematode Caenorhabditis elegans. Genomics, 2004. |
| PubMed ID | 15028283 |
| Short Description | Ethanol-response genes and their regulation analyzed by a microarray and comparative genomic approach in the nematode Caenorhabditis elegans. GEO Record: GSE3727 Platform: GPL2646 GPL2653 GPL3097 Download gene-centric, log2 transformed data: WBPaper00006465.ce.mr.csv |
| # of Conditions | 7 |
Full Description
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The nematode shows responses to acute ethanol exposure that are similar to those observed in humans, mice, and Drosophila, namely hyperactivity followed by uncoordination and sedation. We used in this report the nematode Caenorhabditis elegans as a model system to identify and characterize the genes that are affected by ethanol exposure and to link those genes functionally into an ethanol-induced gene network. By analyzing the expression profiles of all C. elegans ORTs using microarrays, we identified 230 genes affected by ethanol. While the ethanol response of some of the identified genes was significant at early time points, that of the majority was at late time points, indicating that the genes in the latter case might represent the physiological consequence of the ethanol exposure. We further characterized the early response genes that may represent those involved directly in the ethanol response. These genes included many heat shock protein genes, indicating that high concentration of ethanol acts as a strong stress to the animal. Interestingly, we identified two non-heat-shock protein genes that were specifically responsive to ethanol. glr-2 was the only glutamate receptor gene to be induced by ethanol. T28C12.4, which encodes a protein with limited homology to human neuroligin, was also specific to ethanol stress. Finally, by analyzing the promoter regions of the early response genes, we identified a regulatory element, TCTGCGTCTCT, that was necessary for the expression of subsets of ethanol Experimental Details: WBPaper00006465:EtOH_treated_6hr-7 WBPaper00006465:EtOH_treated_6hr-5 WBPaper00006465:EtOH_treated_6hr-3 WBPaper00006465:EtOH_treated_15min-2 WBPaper00006465:EtOH_treated_15min-1 WBPaper00006465:EtOH_treated_30min WBPaper00006465:EtOH_treated_6hr-4. |
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