| Citation | Lee SH, Wong RR, Chin CY, Lim TY, Eng SA, Kong C, Ijap NA, Lau MS, Lim MP, Gan YH, He FL, Tan MW, Nathan S. Burkholderia pseudomallei suppresses Caenorhabditis elegans immunity by specific degradation of a GATA transcription factor. Proc Natl Acad Sci U S A, 2013. |
| PubMed ID | 23980181 |
| Short Description | Burkholderia pseudomallei suppresses Caenorhabditis elegans immunity by specific degradation of a GATA transcription factor. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00044091.ce.mr.csv |
| # of Conditions | 27 |
Full Description
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Burkholderia pseudomallei is a Gram-negative soil bacterium that infects both humans and animals. Although cell culture studies have revealed significant insights into factors contributing to virulence and host defense, the interactions between this pathogen and its intact host remain to be elucidated. To gain insights into the host defense responses to B. pseudomallei infection within an intact host, we analyzed the genome-wide transcriptome of infected Caenorhabditis elegans and identified 6% of the nematode genes that were significantly altered over a 12-h course of infection. An unexpected feature of the transcriptional response to B. pseudomallei was a progressive increase in the proportion of down-regulated genes, of which ELT-2 transcriptional targets were significantly enriched. ELT-2 is an intestinal GATA transcription factor with a conserved role in immune responses. We demonstrate that B. pseudomallei down-regulation of ELT-2 targets is associated with degradation of ELT-2 protein by the host ubiquitin-proteasome system. Degradation of ELT-2 requires the B. pseudomallei type III secretion system. Together, our studies using an intact host provide evidence for pathogen-mediated host immune suppression through the destruction of a host transcription factor. Experimental Details: WBPaper00044091:OP50_0h_rep2 WBPaper00044091:OP50_0h_rep3 WBPaper00044091:OP50_2h_rep1 WBPaper00044091:OP50_2h_rep2 WBPaper00044091:OP50_2h_rep3 WBPaper00044091:OP50_4h_rep1 WBPaper00044091:OP50_4h_rep2 WBPaper00044091:OP50_4h_rep3 WBPaper00044091:OP50_8h_rep1 WBPaper00044091:OP50_8h_rep2 WBPaper00044091:OP50_8h_rep3 WBPaper00044091:OP50_12h_rep1 WBPaper00044091:OP50_12h_rep2 WBPaper00044091:OP50_12h_rep3 WBPaper00044091:BpR15_2h_rep1 WBPaper00044091:BpR15_2h_rep2 WBPaper00044091:BpR15_2h_rep3 WBPaper00044091:BpR15_4h_rep1 WBPaper00044091:BpR15_4h_rep2 WBPaper00044091:BpR15_4h_rep3 WBPaper00044091:BpR15_8h_rep1 WBPaper00044091:BpR15_8h_rep2 WBPaper00044091:BpR15_8h_rep3 WBPaper00044091:BpR15_12h_rep1 WBPaper00044091:BpR15_12h_rep2 WBPaper00044091:BpR15_12h_rep3 WBPaper00044091:OP50_0h_rep1. |
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