| Citation | McElwee J, Bubb K, Thomas JH. Transcriptional outputs of the Caenorhabditis elegans forkhead protein DAF-16. Aging Cell, 2003. |
| PubMed ID | 12882324 |
| Short Description | Transcriptional outputs of the Caenorhabditis elegans forkhead protein DAF-16. GEO Record: GSE3089 Platform: GPL2653 Download gene-centric, log2 transformed data: WBPaper00005896.ce.mr.csv |
| # of Conditions | 4 |
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In Caenorhabditis elegans, the forkhead protein DAF-16 transduces insulin-like signals that regulate larval development and adult lifespan. To identify DAF-16-dependent transcriptional alterations that occur in a long-lived C. elegans strain, we used cDNA microarrays and genomic analysis to identify putative direct and indirect DAF-16 transcriptional target genes. Our analysis suggests that DAF-16 action regulates a wide range of physiological responses by altering the expression of genes involved in metabolism, energy generation and cellular stress responses. Furthermore, we observed a large overlap between DAF-16-dependent transcription and genes normally expressed in the long-lived dauer larval stage. Finally, we examined the in vivo role of 35 of these target genes by RNA-mediated interference and identified one gene encoding a putative protease that is necessary for the daf-2 Age phenotype. Experimental Details: WBPaper00005896:daf-2_4-1_vs_daf-16_4-1 WBPaper00005896:daf-2_3-1_vs_daf-16_3-1 WBPaper00005896:daf-2_1-1_vs_daf-16_1-1 WBPaper00005896:daf-2_2-1_vs_daf-16_2-1. |
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