| Citation | Menzel R, Bogaert T, Achazi R. A systematic gene expression screen of Caenorhabditis elegans cytochrome P450 genes reveals CYP35 as strongly xenobiotic inducible. Arch Biochem Biophys, 2001. |
| PubMed ID | 11697852 |
| Short Description | A systematic gene expression screen of Caenorhabditis elegans cytochrome P450 genes reveals CYP35 as strongly xenobiotic inducible. GEO Record: GSE3680 Platform: GPL2646 GPL2655 GPL3097 Download gene-centric, log2 transformed data: WBPaper00004966.ce.mr.csv |
| # of Conditions | 3 |
Full Description
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The soil nematode Caenorhabditis elegans is one of the simplest animals having the status of a laboratory model. Its genome contains 80 cytochrome P450 genes (CYP). In order to study CYP gene expression in C. elegans mixed stages and synchronized hermaphrodites were exposed to 18 known xenobiotic cytochrome P450 inducers. Messenger RNA expression was detected by DNA arrays and semiquantitative RTPCR. Using subfamily-specific primers, a pooled set of exon-rich CYP fragments could be amplified. In this way it was possible to systematically check the influence of different inducers on CYP expression at the same time. The well-known CYP1A inducers beta -naphthoflavone, PCB52, and lansoprazol were the most active and in particular they strongly induced almost all CYP35 isoforms. A few number of further CYP forms were found to be inducible by other xenobiotics like phenobarbital, atrazine, and clofibrate. In addition, a transgenic C. elegans line expressing CYP under control of the CYP35A2 promoter showed a strong induction of the fusion by beta -naphthoflavone in the intestine. Experimental Details: WBPaper00004966:7a_DMSO_vs_7b_naph WBPaper00004966:4a_DMSO_vs_4b_naph WBPaper00004966:8a_DMSO_vs_8b_naph. |
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