| Citation | Taubert S, Hansen M, van Gilst MR, Cooper SB, Yamamoto KR. The Mediator subunit MDT-15 confers metabolic adaptation to ingested material. PLoS Genet, 2008. |
| PubMed ID | 18454197 |
| Short Description | The Mediator subunit MDT-15 confers metabolic adaptation to ingested material. GEO Record: GSE9720 Platform: GPL5859 Download gene-centric, log2 transformed data: WBPaper00031850.ce.mr.csv |
| # of Conditions | 5 |
Full Description
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In eukaryotes, RNA polymerase II (Pol(II)) dependent gene expression requires accessory factors termed transcriptional coregulators. One coregulator that universally contributes to Pol(II)-dependent transcription is the Mediator, a multisubunit complex that is targeted by many transcriptional regulatory factors. For example, the Caenorhabditis elegans Mediator subunit MDT-15 confers the regulatory actions of the sterol response element binding protein SBP-1 and the nuclear hormone receptor NHR-49 on fatty acid metabolism. Here, we demonstrate that MDT-15 displays a broader spectrum of activities, and that it integrates metabolic responses to materials ingested by C. elegans. Depletion of MDT-15 protein or mutation of the mdt-15 gene abrogated induction of specific detoxification genes in response to certain xenobiotics or heavy metals, rendering these animals hypersensitive to toxin exposure. Intriguingly, MDT-15 appeared to selectively affect stress responses related to ingestion, as MDT-15 functional defects did not abrogate other stress responses, e.g., thermotolerance. Together with our previous finding that MDT-15:NHR-49 regulatory complexes coordinate a sector of the fasting response, we propose a model whereby MDT-15 integrates several transcriptional regulatory pathways to monitor both the availability and quality of ingested materials, including nutrients and xenobiotic compounds. Experimental Details: WBPaper00031850:mdt-15(RNAi)_vs_control_rep1 WBPaper00031850:mdt-15(RNAi)_vs_control_rep2 WBPaper00031850:mdt-15(RNAi)_vs_control_rep3 WBPaper00031850:mdt-15(RNAi)_vs_control_rep4 WBPaper00031850:mdt-15(RNAi)_vs_control_rep5. |
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