SPELL - Nematode

Citation	Lee SJ, Murphy CT, Kenyon C. Glucose shortens the life span of C. elegans by downregulating DAF-16/FOXO activity and aquaporin gene expression. Cell Metab, 2009.
PubMed ID	19883616
Short Description	Glucose shortens the life span of C. elegans by downregulating DAF-16/FOXO activity and aquaporin gene expression. GEO Record: GSE18561 GSE18562 GSE18563 Platform: GPL9450 Download gene-centric, log2 transformed data: WBPaper00035479.ce.mr.csv
# of Conditions	14
Full Description	Many studies have addressed the effect of dietary glycemic index on obesity and diabetes, but little is known about its effect on life span itself. We found that adding a small amount of glucose to the medium (2%) shortened the life span of C. elegans by inhibiting the activities of life span-extending transcription factors that are also inhibited by insulin signaling: the FOXO family member DAF-16 and the heat shock factor HSF-1. This effect involved the downregulation of an aquaporin glycerol channel, aqp-1. We show that changes in glycerol metabolism are likely to underlie the life span-shortening effect of glucose and that aqp-1 may act cell nonautonomously as a feedback regulator in the insulin/IGF-1-signaling pathway. Insulin downregulates similar glycerol channels in mammals, suggesting that this glucose-responsive pathway might be conserved evolutionarily. Together, these findings raise the possibility that a low-sugar diet might have beneficial effects on life span in higher organisms. Experimental Details: WBPaper00035479:daf-2(e1370)_daf-16(RNAi)_vs_daf-2(e1370)_control_rep1 WBPaper00035479:daf-2(e1370)_daf-16(RNAi)_vs_daf-2(e1370)_control_rep2 WBPaper00035479:daf-2(e1370)_daf-16(RNAi)_vs_daf-2(e1370)_control_rep3 WBPaper00035479:daf-2(e1370)_daf-16(RNAi)_vs_daf-2(e1370)_control_rep4 WBPaper00035479:daf-2(e1370)_daf-16(RNAi)_vs_daf-2(e1370)_control_rep5 WBPaper00035479:daf-2(e1370)_daf-16(RNAi)_vs_daf-2(e1370)_control_rep6 WBPaper00035479:daf-2(e1370)_daf-16(RNAi)_vs_daf-2(e1370)_control_rep7 WBPaper00035479:daf-2(e1370)_daf-16(RNAi)_vs_daf-2(e1370)_control_rep8 WBPaper00035479:N2_glucose_vs_N2_control_rep1 WBPaper00035479:N2_glucose_vs_N2_control_rep2 WBPaper00035479:N2_glucose_vs_N2_control_rep3 WBPaper00035479:N2_glucose_vs_N2_control_rep4 WBPaper00035479:N2_glucose_vs_N2_control_rep5 WBPaper00035479:N2_glucose_vs_N2_control_rep6.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: aging