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Citation Dinkova TD, Keiper BD, Korneeva NL, Aamodt EJ, Rhoads RE. Translation of a small subset of Caenorhabditis elegans mRNAs is dependent on a specific eukaryotic translation initiation factor 4E isoform. Mol Cell Biol, 2005.
PubMed ID 15601834
Short Description Translation of a small subset of Caenorhabditis elegans mRNAs is dependent on a specific eukaryotic translation initiation factor 4E isoform.
GEO Record: N.A. Platform: N.A.
Download gene-centric, log2 transformed data: WBPaper00024654.ce.mr.csv
# of Conditions 18
Full Description 1316625150_help The mRNA cap-binding protein eukaryotic translation initiation factor 4E (eIF4E) participates in protein synthesis initiation, translational repression of specific mRNAs, and nucleocytoplasmic shuttling. Multiple isoforms of eIF4E are expressed in a variety of organisms, but their specific roles are poorly understood. We investigated one Caenorhabditis elegans isoform, IFE-4, which has homologues in plants and mammals. IFE-4::green fluorescent protein (GFP) was expressed in pharyngeal and tail neurons, body wall muscle, spermatheca, and vulva. Knockout of ife-4 by RNA interference (RNAi) or a null mutation produced a pleiotropic phenotype that included egg-laying defects. Sedimentation analysis demonstrated that IFE-4, but not IFE-1, was present in 48S initiation complexes, indicating that it participates in protein synthesis initiation. mRNAs affected by ife-4 knockout were determined by DNA microarray analysis of polysomal distribution. Polysome shifts, in the absence of total mRNA changes, were observed for only 33 of the 18,967 C. elegans mRNAs tested, of which a disproportionate number were related to egg laying and were expressed in neurons and/or muscle. Translational regulation was confirmed by reduced levels of DAF-12, EGL-15, and KIN-29. The functions of these proteins can explain some phenotypes observed in ife-4 knockout mutants. These results indicate that translation of a limited subset of mRNAs is dependent on a specific isoform of eIF4E.
Experimental Details:
WBPaper00024654:Heavy_polysomes_ife-4(k320)_1
WBPaper00024654:Heavy_polysomes_ife-4(k320)_2
WBPaper00024654:Heavy_polysomes_ife-4(k320)_3
WBPaper00024654:Heavy_polysomes_N2_1
WBPaper00024654:Heavy_polysomes_N2_2
WBPaper00024654:Heavy_polysomes_N2_3
WBPaper00024654:Light_polysomes_ife-4(k320)_1
WBPaper00024654:Light_polysomes_ife-4(k320)_2
WBPaper00024654:Light_polysomes_ife-4(k320)_3
WBPaper00024654:Light_polysomes_N2_1
WBPaper00024654:Light_polysomes_N2_2
WBPaper00024654:Light_polysomes_N2_3
WBPaper00024654:Total_RNA_ife-4(k320)_1
WBPaper00024654:Total_RNA_ife-4(k320)_2
WBPaper00024654:Total_RNA_ife-4(k320)_3
WBPaper00024654:Total_RNA_N2_1
WBPaper00024654:Total_RNA_N2_2
WBPaper00024654:Total_RNA_N2_3.
Tags 1316625150_help
Method: microarray, Species: Caenorhabditis elegans, Topic: unclassified