| Citation | Ercan S, Dick LL, Lieb JD. The C. elegans dosage compensation complex propagates dynamically and independently of X chromosome sequence. Curr Biol, 2009. |
| PubMed ID | 19853451 |
| Short Description | The C. elegans dosage compensation complex propagates dynamically and independently of X chromosome sequence. GEO Record: GSE16621 Platform: GPL8673 Download gene-centric, log2 transformed data: WBPaper00035408.ce.mr.csv |
| # of Conditions | 9 |
Full Description
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BACKGROUND: The C. elegans dosage compensation complex (DCC) associates with both X chromosomes of XX animals to reduce X-linked transcript levels. Five DCC members are homologous to subunits of the evolutionarily conserved condensin complex, and two noncondensin subunits are required for DCC recruitment to X. RESULTS: We investigated the molecular mechanism of DCC recruitment and spreading along X by examining gene expression and the binding patterns of DCC subunits in different stages of development, and in strains harboring X;autosome (X;A) fusions. We show that DCC binding is dynamically specified according to gene activity during development and that the mechanism of DCC spreading is independent of X chromosome DNA sequence. Accordingly, in X;A fusion strains, DCC binding propagates from X-linked recruitment sites onto autosomal promoters as a function of distance. Quantitative analysis of spreading suggests that the condensin-like subunits spread from recruitment sites to promoters more readily than subunits involved in initial X targeting. CONCLUSIONS: A highly conserved chromatin complex is appropriated to accomplish domain-scale transcriptional regulation during C. elegans development. Unlike X recognition, which is specified partly by DNA sequence, spreading is sequence independent and coupled to transcriptional activity. Similarities to the X recognition and spreading strategies used by the Drosophila DCC suggest mechanisms fundamental to chromosome-scale gene regulation. Experimental Details: WBPaper00035408:N2_totalRNA_rep1 WBPaper00035408:N2_totalRNA_rep2 WBPaper00035408:N2_totalRNA_rep3 WBPaper00035408:YPT41_totalRNA_rep1 WBPaper00035408:YPT41_totalRNA_rep2 WBPaper00035408:YPT41_totalRNA_rep3 WBPaper00035408:YPT47_totalRNA_rep1 WBPaper00035408:YPT47_totalRNA_rep2 WBPaper00035408:YPT47_totalRNA_rep3. |
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