| Citation | Derry WB, Bierings R, van Iersel M, Satkunendran T, Reinke V, Rothman JH. Regulation of developmental rate and germ cell proliferation in Caenorhabditis elegans by the p53 gene network. Cell Death Differ, 2007. |
| PubMed ID | 17186023 |
| Short Description | Regulation of developmental rate and germ cell proliferation in Caenorhabditis elegans by the p53 gene network. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00028948.ce.mr.csv |
| # of Conditions | 6 |
Full Description
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Caenorhabditis elegans CEP-1 activates germline apoptosis in response to genotoxic stress, similar to its mammalian counterpart, tumor suppressor p53. In mammals, there are three p53 family members (p53, p63, and p73) that activate and repress many distinct and overlapping sets of genes, revealing a complex transcriptional regulatory network. Because CEP-1 is the sole p53 family member in C. elegans, analysis of this network is greatly simplified in this organism. We found that CEP-1 functions during normal development in the absence of stress to repress many (331) genes and activate only a few (28) genes. In response to genotoxic stress, 1394 genes are activated and 942 are repressed, many of which contain p53-binding sites. Comparison of the CEP-1 transcriptional network with transcriptional targets of the human p53 family reveals considerable overlap between CEP-1-regulated genes and homologues regulated by human p63 and p53, suggesting a composite p53/p63 action for CEP-1. We found that phg-1, the C. elegans Gas1 (growth arrest-specific 1) homologue, is activated by CEP-1 and is a negative regulator of cell proliferation in the germline in response to genotoxic stress. Further, we find that CEP-1 and PHG-1 mediate the decreased developmental rate and embryonic viability of mutations in the clk-2/TEL2 gene, which regulates lifespan and checkpoint responses.Cell Death and Differentiation advance online publication, 22 December 2006; doi:10.1038/sj.cdd.4402075. Experimental Details: WBPaper00028948:N2_vs_cep-1(gk138)_repA WBPaper00028948:N2_vs_cep-1(gk138)_repB WBPaper00028948:N2_vs_cep-1(gk138)_repC WBPaper00028948:N2+UV_vs_cep-1(gk138)+UV_repA WBPaper00028948:N2+UV_vs_cep-1(gk138)+UV_repB WBPaper00028948:N2+UV_vs_cep-1(gk138)+UV_repC. |
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