SPELL - Nematode

Citation	Swain S, Wren JF, Sturzenbaum SR, Kille P, Morgan AJ, Jager T, Jonker MJ, Hankard PK, Svendsen C, Owen J, Hedley BA, Blaxter M, Spurgeon DJ. Linking toxicant physiological mode of action with induced gene expression changes in Caenorhabditis elegans. BMC Syst Biol, 2010.
PubMed ID	20331876
Short Description	Linking toxicant physiological mode of action with induced gene expression changes in Caenorhabditis elegans. GEO Record: GSE21008 GSE21010 GSE21011 GSE21012 Platform: GPL10238 Download gene-centric, log2 transformed data: WBPaper00036123.ce.mr.csv
# of Conditions	72
Full Description	BACKGROUND: Physiologically based modelling using DEBtox (dynamic energy budget in toxicology) and transcriptional profiling were used in Caenorhabditis elegans to identify how physiological modes of action, as indicated by effects on system level resource allocation were associated with changes in gene expression following exposure to three toxic chemicals: cadmium, fluoranthene (FA) and atrazine (AZ). RESULTS: For Cd, the physiological mode of action as indicated by DEBtox model fitting was an effect on energy assimilation from food, suggesting that the transcriptional response to exposure should be dominated by changes in the expression of transcripts associated with energy metabolism and the mitochondria. While evidence for effect on genes associated with energy production were seen, an ontological analysis also indicated an effect of Cd exposure on DNA integrity and transcriptional activity. DEBtox modelling showed an effect of FA on costs for growth and reproduction (i.e. for production of new and differentiated biomass). The microarray analysis supported this effect, showing an effect of FA on protein integrity and turnover that would be expected to have consequences for rates of somatic growth. For AZ, the physiological mode of action predicted by DEBtox was increased cost for maintenance. The transcriptional analysis demonstrated that this increase resulted from effects on DNA integrity as indicated by changes in the expression of genes chromosomal repair. CONCLUSIONS: Our results have established that outputs from process based models and transcriptomics analyses can help to link mechanisms of action of toxic chemicals with resulting demographic effects. Such complimentary analyses can assist in the categorisation of chemicals for risk assessment purposes. Experimental Details: WBPaper00036123:GE31_0mg_Atrazine_rep4 WBPaper00036123:GE31_0mg_Atrazine_rep5 WBPaper00036123:GE31_0mg_Atrazine_rep6 WBPaper00036123:GE31_0mg_Atrazine_rep7 WBPaper00036123:GE31_0mg_Atrazine_rep8 WBPaper00036123:GE31_5mg_Atrazine_rep1 WBPaper00036123:GE31_5mg_Atrazine_rep2 WBPaper00036123:GE31_5mg_Atrazine_rep3 WBPaper00036123:GE31_5mg_Atrazine_rep4 WBPaper00036123:GE31_5mg_Atrazine_rep5 WBPaper00036123:GE31_25mg_Atrazine_rep1 WBPaper00036123:GE31_25mg_Atrazine_rep2 WBPaper00036123:GE31_25mg_Atrazine_rep3 WBPaper00036123:GE31_25mg_Atrazine_rep4 WBPaper00036123:GE31_25mg_Atrazine_rep5 WBPaper00036123:GE31_75mg_Atrazine_rep1 WBPaper00036123:GE31_75mg_Atrazine_rep2 WBPaper00036123:GE31_75mg_Atrazine_rep5 WBPaper00036123:GE31_150mg_Atrazine_rep1 WBPaper00036123:GE31_150mg_Atrazine_rep2 WBPaper00036123:GE31_150mg_Atrazine_rep3 WBPaper00036123:GE31_150mg_Atrazine_rep4 WBPaper00036123:GE31_150mg_Atrazine_rep5 WBPaper00036123:GE31_0mg_Cadmium_rep1 WBPaper00036123:GE31_0mg_Cadmium_rep2 WBPaper00036123:GE31_0mg_Cadmium_rep3 WBPaper00036123:GE31_0mg_Cadmium_rep4b WBPaper00036123:GE31_0mg_Cadmium_rep5b WBPaper00036123:GE31_10mg_Cadmium_rep1 WBPaper00036123:GE31_10mg_Cadmium_rep2 WBPaper00036123:GE31_10mg_Cadmium_rep3 WBPaper00036123:GE31_10mg_Cadmium_rep4 WBPaper00036123:GE31_20mg_Cadmium_rep1 WBPaper00036123:GE31_20mg_Cadmium_rep2 WBPaper00036123:GE31_20mg_Cadmium_rep3 WBPaper00036123:GE31_20mg_Cadmium_rep4 WBPaper00036123:GE31_40mg_Cadmium_rep1 WBPaper00036123:GE31_40mg_Cadmium_rep2 WBPaper00036123:GE31_40mg_Cadmium_rep3 WBPaper00036123:GE31_40mg_Cadmium_rep4 WBPaper00036123:GE31_40mg_Cadmium_rep5 WBPaper00036123:GE31_60mg_Cadmium_rep1 WBPaper00036123:GE31_60mg_Cadmium_rep2 WBPaper00036123:GE31_60mg_Cadmium_rep3 WBPaper00036123:GE31_60mg_Cadmium_rep4 WBPaper00036123:GE31_60mg_Cadmium_rep5 WBPaper00036123:GE31_0mg_Fluoranthene_rep2b WBPaper00036123:GE31_0mg_Fluoranthene_rep3b WBPaper00036123:GE31_0mg_Fluoranthene_rep4c WBPaper00036123:GE31_0mg_Fluoranthene_rep5c WBPaper00036123:GE31_0mg_Fluoranthene_rep6b WBPaper00036123:GE31_0mg_Fluoranthene_rep7b WBPaper00036123:GE31_0mg_Fluoranthene_rep8b WBPaper00036123:GE31_100mg_Fluoranthene_rep1 WBPaper00036123:GE31_100mg_Fluoranthene_rep2 WBPaper00036123:GE31_100mg_Fluoranthene_rep3 WBPaper00036123:GE31_100mg_Fluoranthene_rep4 WBPaper00036123:GE31_250mg_Fluoranthene_rep1 WBPaper00036123:GE31_250mg_Fluoranthene_rep2 WBPaper00036123:GE31_250mg_Fluoranthene_rep3 WBPaper00036123:GE31_250mg_Fluoranthene_rep4 WBPaper00036123:GE31_250mg_Fluoranthene_rep5 WBPaper00036123:GE31_500mg_Fluoranthene_rep1 WBPaper00036123:GE31_500mg_Fluoranthene_rep2 WBPaper00036123:GE31_500mg_Fluoranthene_rep3 WBPaper00036123:GE31_500mg_Fluoranthene_rep4 WBPaper00036123:GE31_500mg_Fluoranthene_rep5 WBPaper00036123:GE31_1000mg_Fluoranthene_rep1 WBPaper00036123:GE31_1000mg_Fluoranthene_rep2 WBPaper00036123:GE31_1000mg_Fluoranthene_rep3 WBPaper00036123:GE31_1000mg_Fluoranthene_rep4 WBPaper00036123:GE31_1000mg_Fluoranthene_rep5.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: response to chemical