| Citation | Karengera A, Verburg I, Sterken MG, Riksen JAG, Murk AJ, Dinkla IJT. Determining Toxic Potencies of Water-Soluble Contaminants in Wastewater Influents and Effluent Using Gene Expression Profiling in C. elegans as a Bioanalytical Tool. Arch Environ Contam Toxicol, 2022. |
| PubMed ID | 36190544 |
| Short Description | Determining Toxic Potencies of Water-Soluble Contaminants in Wastewater Influents and Effluent Using Gene Expression Profiling in C. elegans as a Bioanalytical Tool. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00064552.ce.mr.csv |
| # of Conditions | 24 |
Full Description
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With chemical analysis, it is impossible to qualify and quantify the toxic potency of especially hydrophilic bioactive contaminants. In this study, we applied the nematode C. elegans as a model organism for detecting the toxic potency of whole influent wastewater samples. Gene expression in the nematode was used as bioanalytical tool to reveal the presence, type and potency of molecular pathways induced by 24-h exposure to wastewater from a hospital (H), nursing home (N), community (C), and influent (I) and treated effluent (E) from a local wastewater treatment plant. Exposure to influent water significantly altered expression of 464 genes, while only two genes were differentially expressed in nematodes treated with effluent. This indicates a significant decrease in bioactive pollutant-load after wastewater treatment. Surface water receiving the effluent did not induce any genes in exposed nematodes. A subset of 209 genes was differentially expressed in all untreated wastewaters, including cytochromes P450 and C-type lectins related to the nematode's xenobiotic metabolism and immune response, respectively. Different subsets of genes responded to particular waste streams making them candidates to fingerprint-specific wastewater sources. This study shows that gene expression profiling in C. elegans can be used for mechanism-based identification of hydrophilic bioactive compounds and fingerprinting of specific wastewaters. More comprehensive than with chemical analysis, it can demonstrate the effective overall removal of bioactive compounds through wastewater treatment. This bioanalytical tool can also be applied in the process of identification of the bioactive compounds via a process of toxicity identification evaluation. Experimental Details: WBPaper00064552:Community_Sample16 WBPaper00064552:Community_Sample17 WBPaper00064552:Community_Sample3 WBPaper00064552:Hospital_Sample13 WBPaper00064552:Hospital_Sample5 WBPaper00064552:Hospital_Sample7 WBPaper00064552:NursingHome_Sample2 WBPaper00064552:NursingHome_Sample21 WBPaper00064552:NursingHome_Sample4 WBPaper00064552:Surface_Sample1 WBPaper00064552:Surface_Sample11 WBPaper00064552:Surface_Sample15 WBPaper00064552:Surface_Sample18 WBPaper00064552:Surface_Sample20 WBPaper00064552:Surface_Sample22 WBPaper00064552:Surface_Sample24 WBPaper00064552:Surface_Sample6 WBPaper00064552:Surface_Sample9 WBPaper00064552:WasteEffluent_Sample12 WBPaper00064552:WasteEffluent_Sample14 WBPaper00064552:WasteEffluent_Sample19 WBPaper00064552:WasteInfluent_Sample10 WBPaper00064552:WasteInfluent_Sample23 WBPaper00064552:WasteInfluent_Sample8. |
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