| Citation | Sterken MG, van Wijk MH, Quamme EC, Riksen JAG, Carnell L, Mathies LD, Davies AG, Kammenga JE, Bettinger JC. Transcriptional analysis of the response of C. elegans to ethanol exposure. Sci Rep, 2021. |
| PubMed ID | 34040055 |
| Short Description | Transcriptional analysis of the response of C. elegans to ethanol exposure. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00061443.ce.mr.csv |
| # of Conditions | 40 |
Full Description
|
Ethanol-induced transcriptional changes underlie important physiological responses to ethanol that are likely to contribute to the addictive properties of the drug. We examined the transcriptional responses of Caenorhabditis elegans across a timecourse of ethanol exposure, between 30 min and 8 h, to determine what genes and genetic pathways are regulated in response to ethanol in this model. We found that short exposures to ethanol (up to 2 h) induced expression of metabolic enzymes involved in metabolizing ethanol and retinol, while longer exposure (8 h) had much more profound effects on the transcriptome. Several genes that are known to be involved in the physiological response to ethanol, including direct ethanol targets, were regulated at 8 h of exposure. This longer exposure to ethanol also resulted in the regulation of genes involved in cilia function, which is consistent with an important role for the effects of ethanol on cilia in the deleterious effects of chronic ethanol consumption in humans. Finally, we found that food deprivation for an 8-h period induced gene expression changes that were somewhat ameliorated by the presence of ethanol, supporting previous observations that worms can use ethanol as a calorie source. Experimental Details: WBPaper00061443:0.4mM-ethanol_120min_Sample1 WBPaper00061443:0.4mM-ethanol_480min_Sample2 WBPaper00061443:0.4mM-ethanol_120min_Sample3 WBPaper00061443:0.4mM-ethanol_0min_Sample4 WBPaper00061443:0.4mM-ethanol_60min_Sample5 WBPaper00061443:0.4mM-ethanol_30min_Sample6 WBPaper00061443:0.4mM-ethanol_30min_Sample7 WBPaper00061443:0.4mM-ethanol_60min_Sample8 WBPaper00061443:control_0min_Sample9 WBPaper00061443:control_0min_Sample10 WBPaper00061443:control_480min_Sample11 WBPaper00061443:control_30min_Sample12 WBPaper00061443:control_60min_Sample13 WBPaper00061443:0.4mM-ethanol_480min_Sample14 WBPaper00061443:control_480min_Sample15 WBPaper00061443:0.4mM-ethanol_0min_Sample16 WBPaper00061443:control_60min_Sample17 WBPaper00061443:control_120min_Sample18 WBPaper00061443:control_30min_Sample19 WBPaper00061443:control_120min_Sample20 WBPaper00061443:0.4mM-ethanol_480min_Sample21 WBPaper00061443:0.4mM-ethanol_60min_Sample22 WBPaper00061443:control_60min_Sample23 WBPaper00061443:0.4mM-ethanol_30min_Sample24 WBPaper00061443:0.4mM-ethanol_480min_Sample25 WBPaper00061443:control_120min_Sample26 WBPaper00061443:0.4mM-ethanol_0min_Sample27 WBPaper00061443:control_60min_Sample28 WBPaper00061443:control_120min_Sample29 WBPaper00061443:0.4mM-ethanol_30min_Sample30 WBPaper00061443:control_0min_Sample31 WBPaper00061443:0.4mM-ethanol_60min_Sample32 WBPaper00061443:0.4mM-ethanol_0min_Sample33 WBPaper00061443:control_480min_Sample34 WBPaper00061443:0.4mM-ethanol_120min_Sample35 WBPaper00061443:0.4mM-ethanol_120min_Sample36 WBPaper00061443:control_30min_Sample37 WBPaper00061443:control_0min_Sample38 WBPaper00061443:control_30min_Sample39 WBPaper00061443:control_480min_Sample40. |
|
Tags
|
Contact: help (at) wormbase.org
