| Citation | Lee RC, Hammell CM, Ambros V. Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans. RNA, 2006. |
| PubMed ID | 16489184 |
| Short Description | Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00027111.ce.mr.csv |
| # of Conditions | 21 |
Full Description
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C. elegans contains numerous small RNAs of ~21-24 nt in length. The microRNAs (miRNAs) are small noncoding RNAs produced by DCR-1- and ALG-dependent processing of self-complementary hairpin transcripts. Endogenous small interfering RNAs (endo-siRNAs), associated with ongoing silencing of protein-coding genes in normal worms, are produced by mechanisms that involve DCR-1 but, unlike miRNAs, also involve RDE-2, RDE-3, RDE-4, RRF-1, and RRF-3. The tiny noncoding (tncRNAs) are similar to endo-siRNAs in their biogenesis except that they are derived from noncoding sequences. These endo-siRNA- and tncRNA-based endogenous RNAi pathways involve some components, including DCR-1 and RDE-4, that are shared with exogenous RNAi, and some components, including RRF-3 and ERI-1, that are specific to endogenous RNAi. rrf-3 and eri-1 mutants are enhanced for some silencing processes and defective for others, suggesting cross-regulatory interactions between RNAi pathways in C. elegans. Microarray expression profiling of RNAi-defective mutant worms further suggests diverse endogenous RNAi pathways for silencing different sets of genes. Experimental Details: WBPaper00027111:eri-1(mg366)_1 WBPaper00027111:eri-1(mg366)_2 WBPaper00027111:eri-1(mg366)_3 WBPaper00027111:N2_1 WBPaper00027111:N2_2 WBPaper00027111:N2_3 WBPaper00027111:rde-3(ne298)_1 WBPaper00027111:rde-3(ne298)_2 WBPaper00027111:rde-3(ne298)_3 WBPaper00027111:rde-3(r459)_1 WBPaper00027111:rde-3(r459)_2 WBPaper00027111:rde-3(r459)_3 WBPaper00027111:rrf-1(pk1417)_1 WBPaper00027111:rrf-1(pk1417)_2 WBPaper00027111:rrf-1(pk1417)_3 WBPaper00027111:rrf-2(ok210)_1 WBPaper00027111:rrf-2(ok210)_2 WBPaper00027111:rrf-2(ok210)_3 WBPaper00027111:rrf-3(pk1426)_1 WBPaper00027111:rrf-3(pk1426)_2 WBPaper00027111:rrf-3(pk1426)_3. |
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