| Citation | Vasquez-Rifo A, Jannot G, Armisen J, Labouesse M, Bukhari SI, Rondeau EL, Miska EA, Simard MJ. Developmental characterization of the microRNA-specific C. elegans Argonautes alg-1 and alg-2. PLoS One, 2012. |
| PubMed ID | 22448270 |
| Short Description | Developmental characterization of the microRNA-specific C. elegans Argonautes alg-1 and alg-2. GEO Record: GSE35505 Platform: GPL15181 Download gene-centric, log2 transformed data: WBPaper00040911.ce.mr.csv |
| # of Conditions | 16 |
Full Description
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The genes alg-1 and alg-2 (referred to as "alg-1/2") encode the Argonaute proteins affiliated to the microRNA (miRNA) pathway in C. elegans. Bound to miRNAs they form the effector complex that effects post-transcriptional gene silencing. In order to define biological features important to understand the mode of action of these Argonautes, we characterize aspects of these genes during development. We establish that alg-1/2 display an overlapping spatio-temporal expression profile and shared association to a miRNAs set, but with gene-specific predominant expression in various cells and increased relative association to defined miRNAs. Congruent with their spatio-temporal coincidence and regardless of alg-1/2 drastic post-embryonic differences, only loss of both genes leads to embryonic lethality. Embryos without zygotic alg-1/2 predominantly arrest during the morphogenetic process of elongation with defects in the epidermal-muscle attachment structures. Altogether our results highlight similarities and specificities of the alg-1/2 likely to be explained at different cellular and molecular levels. Experimental Details: WBPaper00040911:L4_ALG-1_IP WBPaper00040911:L4_ALG-2-TAG_IP WBPaper00040911:L4_ALG-1_control_IP WBPaper00040911:L4_ALG-2-TAG_control_IP WBPaper00040911:L3_ALG-1_IP WBPaper00040911:L3_ALG-2-TAG_IP WBPaper00040911:L3_ALG-1_control_IP WBPaper00040911:L3_ALG-2-TAG_control_IP WBPaper00040911:L2_ALG-1_IP WBPaper00040911:L2_ALG-2-TAG_IP WBPaper00040911:L2_ALG-1_control_IP WBPaper00040911:L2_ALG-2-TAG_control_IP WBPaper00040911:L1_ALG-1_IP WBPaper00040911:L1_ALG-2-TAG_IP WBPaper00040911:L1_ALG-1_control_IP WBPaper00040911:L1_ALG-2-TAG_control_IP. |
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