SPELL - Nematode

Citation	Isik M, Blackwell TK, Berezikov E. MicroRNA mir-34 provides robustness to environmental stress response via the DAF-16 network in C. elegans. Sci Rep, 2016.
PubMed ID	27905558
Short Description	MicroRNA mir-34 provides robustness to environmental stress response via the DAF-16 network in C. elegans. GEO Record: GSE76413 Platform: GPL10094 Download gene-centric, log2 transformed data: WBPaper00050488.ce.mr.csv
# of Conditions	30
Full Description	Diverse stresses and aging alter expression levels of microRNAs, suggesting a role for these posttranscriptional regulators of gene expression in stress modulation and longevity. Earlier studies demonstrated a central role for the miR-34 family in promoting cell cycle arrest and cell death following stress in human cells. However, the biological significance of this response was unclear. Here we show that in C. elegans mir-34 upregulation is necessary for developmental arrest, correct morphogenesis, and adaptation to a lower metabolic state to protect animals against stress-related damage. Either deletion or overexpression of mir-34 lead to an impaired stress response, which can largely be explained by perturbations in DAF-16/FOXO target gene expression. We demonstrate that mir-34 expression is regulated by the insulin signaling pathway via a negative feedback loop between miR-34 and DAF-16/FOXO. We propose that mir-34 provides robustness to stress response programs by controlling noise in the DAF-16/FOXO-regulated gene network. Experimental Details: WBPaper00050488:N2_adult_20C_rep1 WBPaper00050488:N2_adult_20C_rep2 WBPaper00050488:N2_adult_20C_rep3 WBPaper00050488:N2_adult_25C_rep1 WBPaper00050488:N2_adult_25C_rep2 WBPaper00050488:N2_adult_25C_rep3 WBPaper00050488:mir-34(gk437)_adult_20C_rep1 WBPaper00050488:mir-34(gk437)_adult_20C_rep2 WBPaper00050488:mir-34(gk437)_adult_20C_rep3 WBPaper00050488:mir-34(gk437)_adult_25C_rep1 WBPaper00050488:mir-34(gk437)_adult_25C_rep2 WBPaper00050488:mir-34(gk437)_adult_25C_rep3 WBPaper00050488:mir-34(OverExpression)_adult_20C_rep1 WBPaper00050488:mir-34(OverExpression)_adult_20C_rep2 WBPaper00050488:mir-34(OverExpression)_adult_25C_rep1 WBPaper00050488:mir-34(OverExpression)_adult_25C_rep2 WBPaper00050488:mir-34(OverExpression)_adult_25C_rep3 WBPaper00050488:N2_dauer_20C_rep1 WBPaper00050488:N2_dauer_20C_rep2 WBPaper00050488:mir-34(gk437)_dauer_20C_rep1 WBPaper00050488:mir-34(gk437)_dauer_20C_rep2 WBPaper00050488:mir-34(OverExpression)_dauer_20C_rep1 WBPaper00050488:daf-2(e1370)_dauer_25C_rep1 WBPaper00050488:daf-2(e1370)_dauer_25C_rep2 WBPaper00050488:daf-2(e1370);mir-34(gk437)_dauer_25C_rep1 WBPaper00050488:daf-2(e1370);mir-34(gk437)_dauer_25C_rep2 WBPaper00050488:mir-34(gk437)_dauer_20C_rep3 WBPaper00050488:mir-34(OverExpression)_dauer_20C_rep2 WBPaper00050488:mir-34(OverExpression)_dauer_20C_rep3 WBPaper00050488:daf-2(e1370);mir-34(gk437)_dauer_25C_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: dauer larval development