| Citation | Studencka M, Wesolowski R, Opitz L, Salinas-Riester G, Wisniewski JR, Jedrusik-Bode M. Transcriptional repression of Hox genes by C. elegans HP1/HPL and H1/HIS-24. PLoS Genet, 2012. |
| PubMed ID | 23028351 |
| Short Description | Transcriptional repression of Hox genes by C. elegans HP1/HPL and H1/HIS-24. GEO Record: GSE33339 Platform: GPL7727 Download gene-centric, log2 transformed data: WBPaper00041609.ce.mr.csv |
| # of Conditions | 8 |
Full Description
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Elucidation of the biological role of linker histone (H1) and heterochromatin protein 1 (HP1) in mammals has been difficult owing to the existence of a least 11 distinct H1 and three HP1 subtypes in mice. Caenorhabditis elegans possesses two HP1 homologues (HPL-1 and HPL-2) and eight H1 variants. Remarkably, one of eight H1 variants, HIS-24, is important for C. elegans development. Therefore we decided to analyse in parallel the transcriptional profiles of HIS-24, HPL-1/-2 deficient animals, and their phenotype, since hpl-1, hpl-2, and his-24 deficient nematodes are viable. Global transcriptional analysis of the double and triple mutants revealed that HPL proteins and HIS-24 play gene-specific roles, rather than a general repressive function. We showed that HIS-24 acts synergistically with HPL to allow normal reproduction, somatic gonad development, and vulval cell fate decision. Furthermore, the hpl-2; his-24 double mutant animals displayed abnormal development of the male tail and ectopic expression of C. elegans HOM-C/Hox genes (egl-5 and mab-5), which are involved in the developmental patterning of male mating structures. We found that HPL-2 and the methylated form of HIS-24 specifically interact with the histone H3 K27 region in the trimethylated state, and HIS-24 associates with the egl-5 and mab-5 genes. Our results establish the interplay between HPL-1/-2 and HIS-24 proteins in the regulation of positional identity in C. elegans males. Experimental Details: WBPaper00041609:Hpl2_His24_rep1 WBPaper00041609:Hpl2_His24_rep2 WBPaper00041609:Hpl1_His24_rep1 WBPaper00041609:Hpl1_His24_rep2 WBPaper00041609:Hpl1_Hpl2_rep1 WBPaper00041609:Hpl1_Hpl2_rep2 WBPaper00041609:Hpl1_Hpl2_His24_rep1 WBPaper00041609:Hpl1_Hpl2_His24_rep2. |
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