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Citation Gamerdinger M, Hanebuth MA, Frickey T, Deuerling E. The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum. Science, 2015.
PubMed ID 25859040
Short Description The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum.
GEO Record: GSE63928 Platform: GPL19516
Download gene-centric, log2 transformed data: WBPaper00046643.ce.mr.csv
# of Conditions 8
Full Description 1316625150_help The sorting of proteins to the appropriate compartment is one of the most fundamental cellular processes. We found that in the model organism Caenorhabditis elegans, correct cotranslational endoplasmic reticulum (ER) transport required the suppressor activity of the nascent polypeptide-associated complex (NAC). NAC did not affect the accurate targeting of ribosomes to ER translocons mediated by the signal recognition particle (SRP) pathway but inhibited additional unspecific contacts between ribosomes and translocons by blocking their autonomous binding affinity. NAC depletion shortened the life span of Caenorhabditis elegans, caused global mistargeting of translating ribosomes to the ER, and provoked incorrect import of mitochondrial proteins into the ER lumen, resulting in a strong impairment of protein homeostasis in both compartments. Thus, the antagonistic targeting activity of NAC is important in vivo to preserve the robustness and specificity of cellular protein-sorting routes.
Experimental Details:
Tags 1316625150_help
Method: microarray, Species: Caenorhabditis elegans, Topic: RNAi gene function study