| Citation | Urano F, Calfon M, Yoneda T, Yun C, Kiraly M, Clark SG, Ron D. A survival pathway for Caenorhabditis elegans with a blocked unfolded protein response. J Cell Biol, 2002. |
| PubMed ID | 12186849 |
| Short Description | A survival pathway for Caenorhabditis elegans with a blocked unfolded protein response. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00005432.ce.mr.csv |
| # of Conditions | 2 |
Full Description
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The unfolded protein response (UPR) counteracts stress caused by unprocessed ER client proteins. A genome-wide survey showed impaired induction of many UPR target genes in xbp-1 mutant Caenorhabditis elegans that are unable to signal in the highly conserved IRE1-dependent UPR pathway. However a family of genes, abu (activated in blocked UPR), was induced to higher levels in ER-stressed xbp-1 mutant animals than in ER-stressed wild-type animals. RNA-mediated interference (RNAi) inactivation of a representative abu family member, abu-1 (AC3.3), activated the ER stress marker hsp-4:.gfp in otherwise normal animals and killed 50% of ER-stressed ire-1 and xbp-1 mutant animals. Abu-1(RNAi) also enhanced the effect of inactivation of sel-1, an ER-associated protein degradation gene. The nine abu genes encode highly related type I transmembrane proteins whose lumenal domains have sequence similarity to a mammalian cell surface scavenger receptor of endothelial cells that binds chemically modified extracellular proteins and directs their lysosomal degradation. Our findings that ABU-1 is an intracellular protein located within the endomembrane system that is induced by ER stress in xbp-1 mutant animals suggest that ABU proteins may interact with abnormal ER client proteins and this function may be particularly important in animals with an impaired UPR. Experimental Details: [cgc5432]:WT [cgc5432]:xbp-1. |
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