SPELL - Nematode

Citation	van Wolfswinkel JC, Claycomb JM, Batista PJ, Mello CC, Berezikov E, Ketting RF. CDE-1 affects chromosome segregation through uridylation of CSR-1-bound siRNAs. Cell, 2009.
PubMed ID	19804759
Short Description	CDE-1 affects chromosome segregation through uridylation of CSR-1-bound siRNAs. GEO Record: GSE18202 Platform: GPL2875 Download gene-centric, log2 transformed data: WBPaper00035269.ce.mr.csv
# of Conditions	4
Full Description	We have studied the function of a conserved germline-specific nucleotidyltransferase protein, CDE-1, in RNAi and chromosome segregation in C. elegans. CDE-1 localizes specifically to mitotic chromosomes in embryos. This localization requires the RdRP EGO-1, which physically interacts with CDE-1, and the Argonaute protein CSR-1. We found that CDE-1 is required for the uridylation of CSR-1 bound siRNAs, and that in the absence of CDE-1 these siRNAs accumulate to inappropriate levels, accompanied by defects in both meiotic and mitotic chromosome segregation. Elevated siRNA levels are associated with erroneous gene silencing, most likely through the inappropriate loading of CSR-1 siRNAs into other Argonaute proteins. We propose a model in which CDE-1 restricts specific EGO-1-generated siRNAs to the CSR-1 mediated, chromosome associated RNAi pathway, thus separating it from other endogenous RNAi pathways. The conserved nature of CDE-1 suggests that similar sorting mechanisms may operate in other animals, including mammals. Experimental Details: WBPaper00035269:N2_vs_cde-1_young_adults WBPaper00035269:cde-1_vs_N2_young_adults WBPaper00035269:N2_vs_cde-1_aged_adults WBPaper00035269:cde-1_vs_N2_aged_adults.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: WT vs. mutant