Citation | Jo H, Shim J, Lee JH, Lee J, Kim JB. IRE-1 and HSP-4 contribute to energy homeostasis via fasting-induced lipases in C. elegans. Cell Metab, 2009. |
PubMed ID | 19416714 |
Short Description | IRE-1 and HSP-4 contribute to energy homeostasis via fasting-induced lipases in C. elegans. GEO Record: GSE15656 Platform: GPL7272 Download gene-centric, log2 transformed data: WBPaper00033126.ce.mr.csv |
# of Conditions | 1 |
Full Description
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The endoplasmic reticulum (ER) is an organelle associated with lipid metabolism. However, the involvement of the ER in nutritional status-dependent energy homeostasis is largely unknown. We demonstrate that IRE-1, an ER protein known to be involved in the unfolded protein response, and HSP-4, an ER chaperone, regulate expression of the novel fasting-induced lipases FIL-1 and FIL-2, which induce fat granule hydrolysis upon fasting in C. elegans. RNAi and ectopic expression experiments demonstrated that FIL-1 and FIL-2 are both necessary and sufficient for fasting-induced fat granule breakdown. Failure of ire-1 and hsp-4 mutant animals to hydrolyze fat granules during starvation impaired their motility, which was rescued by glucose supplementation, implicating the importance of ire-1/hsp-4-dependent lipolysis for energy supply from stored fat during fasting. These data suggest that the ER-resident proteins IRE-1 and HSP-4 are key nutritional sensors that modulate expression of inducible lipases to maintain whole-body energy homeostasis in C. elegans. Experimental Details: WBPaper00033126:synchronized_L4_fasted. |
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