| Citation | Iser WB, Wilson MA, Wood WH, Becker K, Wolkow CA. Co-regulation of the DAF-16 target gene, cyp-35B1/dod-13, by HSF-1 in C. elegans dauer larvae and daf-2 insulin pathway mutants. PLoS One, 2011. |
| PubMed ID | 21408062 |
| Short Description | Co-regulation of the DAF-16 target gene, cyp-35B1/dod-13, by HSF-1 in C. elegans dauer larvae and daf-2 insulin pathway mutants. GEO Record: GSE18200 Platform: GPL2875 Download gene-centric, log2 transformed data: WBPaper00038237.ce.mr.csv |
| # of Conditions | 8 |
Full Description
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Insulin/IGF-I-like signaling (IIS) has both cell autonomous and non-autonomous functions. In some cases, targets through which IIS regulates cell-autonomous functions, such as cell growth and metabolism, have been identified. In contrast, targets for many non-autonomous IIS functions, such as C. elegans dauer morphogenesis, remain elusive. Here, we report the use of genomic and genetic approaches to identify potential non-autonomous targets of C. elegans IIS. First, we used transcriptional microarrays to identify target genes regulated non-autonomously by IIS in the intestine or in neurons. C. elegans IIS controls expression of a number of stress response genes, which were differentially regulated by tissue-restricted IIS. In particular, expression of sod-3, a MnSOD enzyme, was not regulated by tissue-restricted IIS on the microarrays, while expression of hsp-16 genes was rescued back to wildtype by tissue restricted IIS. One IIS target regulated non-autonomously by age-1 was cyp-35B1/dod-13, encoding a cytochrome P450. Genetic analysis of the cyp-35B1 promoter showed both DAF-16 and HSF-1 are direct regulators. Based on these findings, we propose that hsf-1 may participate in the pathways mediating non-autonomous activities of age-1 in C. elegans. Experimental Details: WBPaper00038237:age-1(mg44)_rep1 WBPaper00038237:intestine_age-1_rep1 WBPaper00038237:neuron_age-1_rep1 WBPaper00038237:age-1(mg44)_rep2 WBPaper00038237:intestine_age-1_rep2 WBPaper00038237:neuron_age-1_rep2 WBPaper00038237:intestine_age-1_rep3 WBPaper00038237:neuron_age-1_rep3. |
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