SPELL - Nematode

Citation	Egge N, Arneaud SLB, Fonseca RS, Zuurbier KR, McClendon J, Douglas PM. Trauma-induced regulation of VHP-1 modulates the cellular response to mechanical stress. Nat Commun, 2021.
PubMed ID	33674585
Short Description	Trauma-induced regulation of VHP-1 modulates the cellular response to mechanical stress. GEO Record: GSE148325 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00061115.ce.mr.csv
# of Conditions	15
Full Description	Mechanical stimuli initiate adaptive signal transduction pathways, yet exceeding the cellular capacity to withstand physical stress results in death. The molecular mechanisms underlying trauma-induced degeneration remain unclear. In the nematode C. elegans, we have developed a method to study cellular degeneration in response to mechanical stress caused by blunt force trauma. Herein, we report that physical injury activates the c-Jun kinase, KGB-1, which modulates response elements through the AP-1 transcriptional complex. Among these, we have identified a dual-specificity MAPK phosphatase, VHP-1, as a stress-inducible modulator of neurodegeneration. VHP-1 regulates the transcriptional response to mechanical stress and is itself attenuated by KGB-1-mediated inactivation of a deubiquitinase, MATH-33, and proteasomal degradation. Together, we describe an uncharacterized stress response pathway in C. elegans and identify transcriptional and post-translational components comprising a feedback loop on Jun kinase and phosphatase activity. Experimental Details: WBPaper00061115:Day1Adult_uninjured_rep1 WBPaper00061115:Day1Adult_uninjured_rep2 WBPaper00061115:Day1Adult_uninjured_rep3 WBPaper00061115:Day1Adult_injured_rep1 WBPaper00061115:Day1Adult_injured_rep2 WBPaper00061115:Day1Adult_injured_rep3 WBPaper00061115:Day4Adult_uninjured_rep1 WBPaper00061115:Day4Adult_uninjured_rep2 WBPaper00061115:Day4Adult_uninjured_rep3 WBPaper00061115:Day4Adult_injured_rep1 WBPaper00061115:Day4Adult_injured_rep2 WBPaper00061115:Day4Adult_injured_rep3 WBPaper00061115:Day4Adult_injured-4x_rep1 WBPaper00061115:Day4Adult_injured-4x_rep2 WBPaper00061115:Day4Adult_injured-4x_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: unclassified