SPELL - Nematode

Citation	Baugh LR, Hill AA, Claggett JM, Hill-Harfe K, Wen JC, Slonim DK, Brown EL, Hunter CP. The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo. Development, 2005.
PubMed ID	15772128
Short Description	The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo. GEO Record: GSE2180 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00025032.ce.mr.csv
# of Conditions	30
Full Description	Maternal and zygotic activities of the homeodomain protein PAL-1 specify the identity and maintain the development of the multipotent C blastomere lineage in the C. elegans embryo. To identify PAL-1 regulatory target genes, we used microarrays to compare transcript abundance in wild-type embryos with mutant embryos lacking a C blastomere and to mutant embryos with extra C blastomeres. pal-1-dependent C-lineage expression was verified for select candidate target genes by reporter gene analysis, though many of the target genes are expressed in additional lineages as well. The set of validated target genes includes 12 transcription factors, an uncharacterized wingless ligand and five uncharacterized genes. Phenotypic analysis demonstrates that the identified PAL-1 target genes affect specification, differentiation and morphogenesis of C-lineage cells. In particular, we show that cell fate-specific genes (or tissue identity genes) and a posterior HOX gene are activated in lineage-specific fashion. Transcription of targets is initiated in four temporal phases, which together with their spatial expression patterns leads to a model of the regulatory network specified by PAL-1. Experimental Details: WBPaper00025032:mex-3_skn-1_0_min WBPaper00025032:mex-3_skn-1_101_min WBPaper00025032:mex-3_skn-1_122_min WBPaper00025032:mex-3_skn-1_143_min WBPaper00025032:mex-3_skn-1_186_min WBPaper00025032:mex-3_skn-1_23_min WBPaper00025032:mex-3_skn-1_41_min WBPaper00025032:mex-3_skn-1_53_min WBPaper00025032:mex-3_skn-1_66_min WBPaper00025032:mex-3_skn-1_83_min WBPaper00025032:N2_0_min WBPaper00025032:N2_101_min WBPaper00025032:N2_122_min WBPaper00025032:N2_143_min WBPaper00025032:N2_186_min WBPaper00025032:N2_23_min WBPaper00025032:N2_41_min WBPaper00025032:N2_53_min WBPaper00025032:N2_66_min WBPaper00025032:N2_83_min WBPaper00025032:pie-1_0_min WBPaper00025032:pie-1_101_min WBPaper00025032:pie-1_122_min WBPaper00025032:pie-1_143_min WBPaper00025032:pie-1_186_min WBPaper00025032:pie-1_23_min WBPaper00025032:pie-1_41_min WBPaper00025032:pie-1_53_min WBPaper00025032:pie-1_66_min WBPaper00025032:pie-1_83_min.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: WT vs. mutant