| Citation | Eckl J, Sima S, Marcus K, Lindemann C, Richter K. Hsp90-downregulation influences the heat-shock response, innate immune response and onset of oocyte development in nematodes. PLoS One, 2017. |
| PubMed ID | 29078207 |
| Short Description | Hsp90-downregulation influences the heat-shock response, innate immune response and onset of oocyte development in nematodes. GEO Record: GSE105030 Platform: GPL19230 Download gene-centric, log2 transformed data: WBPaper00053254.ce.mr.csv |
| # of Conditions | 6 |
Full Description
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Hsp90 is a molecular chaperone involved in the regulation and maturation of kinases and transcription factors. In Caenorhabditis elegans, it contributes to the development of fertility, maintenance of muscle structure, the regulation of heat-shock response and dauer state. To understand the consequences of Hsp90-depletion, we studied Hsp90 RNAi-treated nematodes by DNA microarrays and mass spectrometry. We find that upon development of phenotypes the levels of chaperones and Hsp90 cofactors are increased, while specific proteins related to the innate immune response are depleted. In microarrays, we further find many differentially expressed genes related to gonad and larval development. These genes form an expression cluster that is regulated independently from the immune response implying separate pathways of Hsp90-involvement. Using fluorescent reporter strains for the differentially expressed immune response genes skr-5, dod-24 and clec-60 we observe that their activity in intestinal tissues is influenced by Hsp90-depletion. Instead, effects on the development are evident in both gonad arms. After Hsp90-depletion, changes can be observed in early embryos and adults containing fluorescence-tagged versions of SEPA-1, CAV-1 or PUD-1, all of which are downregulated after Hsp90-depletion. Our observations identify molecular events for Hsp90-RNAi induced phenotypes during development and immune responses, which may help to separately investigate independent Hsp90-influenced processes that are relevant during the nematode's life and development. Experimental Details: WBPaper00053254:Control_rep1 WBPaper00053254:Control_rep2 WBPaper00053254:Control_rep3 WBPaper00053254:hsp-90(RNAi)_rep1 WBPaper00053254:hsp-90(RNAi)_rep2 WBPaper00053254:hsp-90(RNAi)_rep3. |
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