| Citation | Hu CC, Wu GH, Hua TE, Wagner OI, Yen TJ. Uptake of TiO2 Nanoparticles into C. elegans Neurons Negatively Affects Axonal Growth and Worm Locomotion Behavior. ACS Appl Mater Interfaces, 2018. |
| PubMed ID | 29464946 |
| Short Description | Uptake of TiO2 Nanoparticles into C. elegans Neurons Negatively Affects Axonal Growth and Worm Locomotion Behavior. GEO Record: GSE95603 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00053737.ce.mr.csv |
| # of Conditions | 2 |
Full Description
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We employ the model organism Caenorhabditis elegans to effectively study the toxicology of anatase and rutile phase titanium dioxide (TiO2) nanoparticles (NPs). The experimental results show that the nematode C. elegans can take up fluorescein isothiocyanate (FITC)-labeled TiO2 NPs and that both anatase and rutile TiO2 NPs can be detected in the cytoplasm of cultured primary neurons imaged by transmission electron microscopy (TEM). After TiO2 NPs exposure, these neurons also grow shorter axons, which may be related to the detected impeded worm locomotion behavior. Furthermore, anatase TiO2 NPs did not affect the worm's body length; however, we determined that a concentration of 500 g/ml anatase TiO2 NPs reduced a worm population by 50% within 72 hours. Notably, rutile TiO2 NPs negatively affect both body size and worm population. Worms unable to enter the L4 larval stage explain a severe reduction in the worm population at TiO2 NPs LC50/3d. To obtain a better understanding of the cellular mechanisms involved in TiO2 NPs intoxication, DNA microarray assays were employed to determine changes in gene expression in the presence or absence of TiO2 NPs exposure. Our data reveal three genes (with significant changes in expression levels) were related to metal binding or metal detoxification (mtl-2, C45B2.2 and nhr-247), six genes involved in fertility and reproduction (mtl-2, F26F2.3, ZK970.7, clec-70, K08C9.7 and C38C3.7), four genes involved in worm growth and body morphogenesis (mtl-2, F26F2.3, C38C3.7 and nhr-247), and five genes involved in neuronal function (C41G6.13, C45B2.2, srr-6, K08C9.7 and C38C3.7). Experimental Details: WBPaper00053737:Control_rep3 WBPaper00053737:anatase-TiO2_rep3. |
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