| Citation | Kim H, Jeong J, Chatterjee N, Roca CP, Yoon D, Kim S, Kim Y, Choi J. JAK/STAT and TGF-B activation as potential adverse outcome pathway of TiO2NPs phototoxicity in Caenorhabditis elegans. Sci Rep, 2017. |
| PubMed ID | 29259193 |
| Short Description | JAK/STAT and TGF-B activation as potential adverse outcome pathway of TiO2NPs phototoxicity in Caenorhabditis elegans. GEO Record: GSE84441 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00053505.ce.mr.csv |
| # of Conditions | 12 |
Full Description
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Titanium dioxide nanoparticles (TiO2NPs) are widely used nanoparticles, whose catalytic activity is mainly due to photoactivation. In this study, the toxicity of TiO2NPs was investigated on the nematode Caenorhabditis elegans, with and without UV activation. Comparative analyses across the four treatments revealed that UV-activated TiO2NPs led to significant reproductive toxicity through oxidative stress. To understand the underlying molecular mechanism, transcriptomics and metabolomics analyses were conducted, followed by whole-genome network-based pathway analyses. Differential expression analysis from microarray data revealed only 4 DEGs by exposure to TiO2NPs alone, compared to 3,625 and 3,286 DEGs by UV alone and UV-activated TiO2NPs, respectively. Pathway analyses suggested the possible involvement of the JAK/STAT and TGF-B pathways in the phototoxicity of TiO2NPs, which correlated with the observation of increased gene expression of those pathways. Comparative analysis of C. elegans response across UV activation and TiO2NPs exposure was performed using loss-of-function mutants of genes in these pathways. Results indicated that the JAK/STAT pathway was specific to TiO2NPs, whereas the TGF-B pathway was specific to UV. Interestingly, crosstalk between these pathways was confirmed by further mutant analysis. We consider that these findings will contribute to understand the molecular mechanisms of toxicity of TiO2NPs in the natural environment. Experimental Details: WBPaper00053505:Control_rep1 WBPaper00053505:Control_rep2 WBPaper00053505:Control_rep3 WBPaper00053505:UV_rep1 WBPaper00053505:UV_rep2 WBPaper00053505:UV_rep3 WBPaper00053505:TiO2_rep1 WBPaper00053505:TiO2_rep2 WBPaper00053505:TiO2_rep3 WBPaper00053505:UV-TiO2_rep1 WBPaper00053505:UV-TiO2_rep2 WBPaper00053505:UV-TiO2_rep3. |
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